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AssigneeAllergan Sales, Inc.
InventorsLance Steward, Marcella Gilmore, Kei Aoki
Estimating the term…
The present invention provides a nucleic acid molecule which contains a nucleotide sequence encoding a SNAP-25 substrate which includes (i) a green fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a portion of SNAP-25 that includes a BoNT/A, BoNT/C1 or BoNT/E recognition sequence containing a cleavage site, where the cleavage site intervenes between the green fluorescent protein and the first partner of the affinity couple. Further provided herein is a nucleic acid molecule which contains a nucleotide sequence encoding a tagged toxin substrate which includes (i) a fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a clostridial toxin recognition sequence containing a cleavage site, where the cleavage site intervenes between the fluorescent protein and the first partner of the affinity couple.
Method of determining clostridial toxin protease activity, comprising the steps of:
A nucleic acid molecule, comprising a nucleotide sequence that encodes a SNAP-25 substrate comprising
A nucleic acid molecule, comprising a nucleotide sequence encoding a tagged toxin substrate, said tagged toxin substrate comprising:
53 The nucleic acid molecule of claim 52, wherein said fluorescent protein is selected from the group green fluorescent protein (GFP), blue fluorescent protein (BFP), cyan fluorescent protein (CFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP).
The nucleic acid molecule of claim 53, wherein said fluorescent protein is green fluorescent protein (GFP).
Dependencies are inferred from the claim text.