Compounds and methods of early diagnosis of cervical cancer and genital condyloma with hpv, chsp60 tumor suppressor h-ras, k-ras and pten derived peptides modified

An isolated protein sequence or peptide from HPV for detecting or diagnosing cancer or cellular abnormalities, said isolated protein sequence or peptide selected from the group consisting of:

• an E2 region of HPV 18 and an L1 region of HPV 16 and HPV 18 as set forth in SEQ. ID. NO.:
• 9;
• an E7 region of HPV 16 and an L1 region of HPV 16 and HPV 18 as set forth in SEQ. ID. NO.:
• 11;
• an E7 region of HPV 16 and HPV 18 as set forth in SEQ. ID. NO.:
• 12;
• an L1 region of HPV 16 and HPV 18 and an E7 region of HPV 58 as set forth in SEQ. ID. NO.:
• 14;
• an E7 region of HPV 58 and HPV 16 as set forth in SEQ. ID. NO.:
• 15;
• an E6 region of HPV 58 and an E7 region of HPV 16 as set forth in SEQ. ID. No.:
• 16;
• an E6 region of HPV 58 and HPV 16 as set forth in SEQ. ID. No.:
• 17;
• an E7 region of HPV 16 and an E6 region of HPV 58 as set forth in SEQ. ID. No.:
• 18;
• an E6 region of HPV 58 and an E7 region of HPV 16 as set forth in SEQ. ID. No.:
• 19;
• an E7 region of HPV 16 and an E6 region of HPV 58 and HPV 16 as set forth in SEQ. ID. No.:
• 20;
• an E6 region of HPV 16 and HPV 52 and an E7 region of HPV 58 as set forth in SEQ. ID. No.:
• 22;
• an E7 region of HPV 16 and HPV 58 as set forth in SEQ. ID. No.:
• 23;
• an E6 region of HPV 16 and HPV 52 as set forth in SEQ. ID. No.:
• 24;
• an E6 region of HPV 52 and an E7 region of HPV 58 as set forth in SEQ. ID. No.:
• 25;
• an E7 region of HPV 58 and HPV 52 as set forth in SEQ. ID. No.:
• 28;
• an E6 and E2 region of HPV 16 as set forth in SEQ. ID. No.:
• 29;
• an E7 region of HPV 16 as set forth in SEQ. ID. No.:
• 30;
• and
• an E7 and E2 region of HPV 18 as set forth in SEQ. ID. No.:
• 43.

An isolated protein sequence or peptide from H-Ras characterized by a linkage to another isolated protein sequence from HPV for detecting or diagnosing cancer or cellular abnormalities, said isolated protein sequence or peptide selected from the group consisting of:

• a region of H-Ras and an E4 region of HPV 16 as set forth in SEQ. ID. NO.:
• 154;
• a region of H-Ras and an E4 region of HPV 33 as set forth in SEQ. ID. NO.:
• 155;
• a region of H-Ras and an E4 region of HPV 33 as set forth in SEQ. ID. NO.:
• 156;
• and
• a region of H-Ras and an E4 region of HPV 33 as set forth in SEQ. ID. NO.:
• 157.

An isolated protein sequence or peptide from K-Ras characterized by a linkage to another isolated protein sequence from HPV for detecting or diagnosing cancer or cellular abnormalities, said isolated protein sequence or peptide selected from the group consisting of:

• a region of K-Ras and an E2 region of HPV 16 as set forth in SEQ. ID. NO.:
• 165;
• and
• a region of K-Ras and an E5 region of HPV 16 as set forth in SEQ. ID. NO.:
• 167.

An isolated protein sequence or peptide from PTEN for detecting or diagnosing cancer or cellular abnormalities, said isolated protein sequence or peptide selected from the group consisting of:

• a region of PTEN as set forth in SEQ. ID. NO.:
• 174;
• a region of PTEN as set forth in SEQ. ID. NO.:
• 175;
• and
• a region of PTEN as set forth in SEQ. ID. NO.:
• 176.

An isolated protein sequence or peptide from CHSP60 for detecting or diagnosing cancer or cellular abnormalities, said isolated protein sequence or peptide selected from the group consisting of:

• a region of CHSP60 as set forth in SEQ. ID. NO.:
• 170;
• a region of CHSP60 as set forth in SEQ. ID. NO.:
• 171;
• and
• a region of CHSP60 as set forth in SEQ. ID. NO.:
• 172.

An isolated protein sequence or peptide from CHSP60 characterized by a linkage to another isolated protein sequence from HPV for detecting or diagnosing cancer or cellular abnormalities, said isolated protein sequence or peptide selected from the group consisting of:

• a region of CHSP60 and an E7 region of HPV 16 as set forth in SEQ. ID. NO.:
• 178;
• and
• a region of CHSP60 protein and an E6 region of HPV 16 as set forth in SEQ. ID. NO.:
• 179.

A method for detecting or diagnosing cancer or cellular abnormalities, said method comprising the steps of:

• reacting a sample of body fluid or tissue likely to contain antibodies to one or more protein sequences or peptides isolated from the E2, E4, E6, E7 early or late coding region of human papillomavirus (HPV), and characterized by a linkage to another protein sequence or peptide isolated from the E2, E4, E6, E7 early or late coding regions of HPV by a spacer sequence, wherein the isolated protein sequence or peptide consists of more than 50% hydrophilic amino acids, and is recognized by a specific antibody of HPV;
• forming an antibody-peptide complex comprising at least one of the isolated protein sequences or peptides and the sample antibodies;
• and
• detecting the antibody-peptide complex.