Methamphetamine derivatives and conjugates for immunoassay
AssigneeRoche Diagnostics Operatins, Inc.
InventorsRaymond Hui, Stephen Vitone
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Abstract
Compounds of methamphetamine derivatives having a meta-substituted alkyl linker on the benzene ring and a protective group on the nitrogen of the methamphetamine hapten. Such compounds have the structure wherein R1 is an alkyl linker comprising 2-15 carbon atoms and 0-6 heteroatoms, R2 is a leaving group, and R3 is a protecting group.
Claims12
A compound having the formula
- wherein R1 is an alkyl linker comprising 2-15 carbon atoms and 0-6 heteroatoms, R2 is a leaving group, and R3 is a protecting group.
A compound having the formula
- wherein R1 is an alkyl linker comprising 2-15 carbon atoms and 0-6 heteroatoms and R2 is a polysaccharide or a microparticle.
The compound 4-{[4-(3-{(S)-2-[methyl-(2,2,2-trifluoro-acetyl)-amino]-propyl}-phenoxy)-butyrylamino]-methyl}-benzoic acid 2,5-dioxo-pyrrolidin-1-yl ester having the formula
The compound having the formula
An immunoassay for determining methamphetamine in a sample comprising the steps of:
- (a) combining a sample suspected of containing methamphetamine with an antibody specific for methamphetamine and a labeled analyte analog having the structure
- wherein R1 is an alkyl linker comprising 2-15 carbon atoms and 0-6 heteroatoms and R2 is a polysaccharide or a microparticle, whereby the methamphetamine and the analyte analog competitively bind to the antibody, and
- (b) determining the amount of labeled analog bound or unbound to the antibody as a measure of the methamphetamine in the sample.
A test kit for use in determining methamphetamine in a sample comprising
- (a) an antibody specific for methamphetamine and
- (b) a labeled analyte analog having the structure
- wherein R1 is an alkyl linker comprising 2-15 carbon atoms and 0-6 heteroatoms and R2 is a polysaccharide or a microparticle.
Dependencies are inferred from the claim text.