Microfluidic differential extraction cartridge
AssigneeMicroFluidic Systems, Inc.
InventorsBob Yuan, Nima Aflatooni
Life of this patent
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Abstract
A differential extraction system is directed to a microfluidic-based integrated cartridge to automate differential extraction of specific cell types within a mixed sample. The integrated cartridge includes a sonication module for selective cell lysis, separating means to eliminate centrifugation, high surface area pillar chip modules to purify DNA from a cell lysate, and microfluidic circuitry to integrate the steps in an automated platform.
Claims21
A method of selectively lysing a specific cell type, the method comprising:
- a. providing a sample including at least two different cell types;
- b. lysing a first cell type to form a first lysate while at least one remaining cell type remains intact;
- and
- c. separating the first lysate from the sample.
7-46. (canceled)
A method to automate the selective lysing of a specific cell type, the method comprising:
- a. providing a sample including at least two different cell types;
- b. automatically lysing a first cell type to form a first lysate while at least one remaining cell type remains intact;
- and
- c. automatically separating the first lysate from the sample.
Dependencies are inferred from the claim text.