Loading patent…
OwnerAnita Sjogren, Eva Karin Kilmare +2inventors
InventorsAnita Sjogren, Eva Karin Kilmare, Sven Enerback, Peter Eriksson
Estimating the term…
An improved method for vitrification of biological cells, especially blastocyst-derived stem cells (BS cells). The method is very mild for the cells that remain viable after they have been thawed. The method comprises, i) transfer of the cells to a first solution (solution A), ii) optionally incubation of the cells in the first solution, iii) transfer the cells obtained in step i) or ii) to a second solution (solution B), iv) optionally incubation of the cells in the second solution, v) transfer of the cells obtained from step iii) or iv) into one or more closed straws with dimensions that allow a volume of at least 20 μl to be contained in them vi) sealing the one or more closed straws, and vii) vitrification of the one or more closed straws. An important feature of the present invention is the use of closed straw and that relatively large volumes can be efficiently vitrified and subsequently thawed.
A method for vitrification of cells, comprising
A method according to any of the preceding claims, wherein the cells are BS cells or BS cell lines.
A method according to any of the preceding claims, wherein the cells are hBS cells or hBS cell lines.
A method according to any of the preceding claims, wherein at least one of the first and second solutions comprises one or more cryoprotectants.
A method according to any of the preceding claims, wherein at least one of the first and the second solution comprises a viscosity-adjusting agent.
A method according to any of the preceding claims, wherein at least one of the first and second solutions is an aqueous solution.
A method according to any of the preceding claims, wherein step ii) is included.
A method according to any of the preceding claims, wherein step iv) is included.
A method according to any of the preceding claims, wherein about 50% or more such as, e.g., about 55% or more, about 60% or more, about 65% or more, about 70% or more, about 75% or more, about 80% or more, about 85% or more, about 90% or more or about 95% or more of the cells are viable after being devitrified and cultured in a suitable medium.
Dependencies are inferred from the claim text.