Plasmid vector for expression of hepatitis B virus genes in plants, transgenic cell lines for the genes, and the use thereof in manufacture

A transgenic plant cell line comprising one selected from the group consisting of a construct, a vector comprising the construct, or a Agrobacterium tumefaciens comprising the construct, the construct comprising the HBsAg gene, in which a promoter that enables the highly-efficient expression of the gene is assembled at the 5′ end of the HBsAg gene, and a terminator that can enhance the expression of the gene is assembled at the 3′ end of the HBsAg gene, and wherein the cell line is ginseng cell line.

A method for preparing a transgenic plant cell line comprising a construct or a vector comprising the HBsAg gene, comprising:

• 1) introducing into the plant cells a construct or vector comprising the HBsAg gene, in which a promoter that enables the highly-efficient expression of the gene is assembled at the 5′ end of the HBsAg gene, and a terminator which can enhance the expression of the gene is assembled at the 3′ end of the HBsAg gene;
• and
• 2) enabling the plant cells to express the HBsAg proteins.