Reproductive ablation constructs
AssigneeArborgen, LLC
InventorsWilliam Rottmann, Kim Norris-Caneda, Chunscheng Zhang
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Abstract
The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.
Claims83
An isolated polynucleotide selected from the group consisting of SEQ ID NOs:
- 1-8 and 13-26, or functional variants thereof, wherein the sequences depicted in any one of SEQ ID NOs. 1, 2, 3, 4, or 16 confers reproductive-preferred gene expression in a plant cell.
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A construct comprising a polynucleotide selected from any one of SEQ ID NO:
- 1, 2, 3, 4, or 16 or functional variants thereof operably linked to a desired nucleic acid, wherein said polynucleotide regulates the expression of said desired nucleic acid in a plant cell transformed with the construct.
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A method for producing a transgenic plant, comprising (a) transforming a plant cell with a construct that comprises (i) at least one polynucleotide having the sequence of any one of SEQ ID NOs. 1, 2, 3, 4, or 16 or functional variants thereof operably linked to a desired nucleic acid, wherein said polynucleotide regulates the activity of said desired nucleic acid or (ii) a polynucleotide having the sequence depicted in any one of SEQ ID NOs:
- 13, 14, or 15;
- (b) culturing said transformed plant cell under conditions that promote growth of a plant, wherein said plant is a transgenic plant that exhibits a phenotype that is different from a plant of the same species that does not contain said construct.
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An isolated mutant barnase enzyme encoded by a polynucleotide that comprises the sequence depicted in any one of SEQ ID NO:
- 5-8 or variants thereof, wherein the encoded mutant bamase enzyme has attenuated activity compared to a wild-type barnase enzyme.
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A method for obtaining wood pulp, comprising (a) introducing into a plant cell of a woody plant a construct comprising (i) a promoter having the sequence of any one of SEQ ID NOs:
- 1, 2, 3, 4, or 16 or functional variants thereof operably linked to (ii) a desired nucleic acid that comprises the sequence depicted in any one of SEQ ID NOs:
- 5, 6, 7, or 8;
- (b) culturing said transformed plant cell under conditions that promote growth of a plant;
- and (c) obtaining wood pulp from said plant.
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A method for conferring reproductive sterility in a transgenic plant, comprising (a) transforming a plant cell with a construct having a reproductive-preferred promoter operably linked to a cytotoxic gene and a non-reproductive-preferred promoter operably linked to a gene encoding a protein that inhibits said cytotoxic gene;
- wherein said reproductive-preferred promoter is active in an angiosperm or gymnosperm reproductive structure and said non-reproductive-preferred promoter is not active in an angiosperm or gymnosperm reproductive structure;
- (b) culturing said transformed plant cell under conditions that promote growth of a plant;
- and (c) selecting a transgenic plant having an ablated reproductive structure, wherein said reproductive-preferred promoters are selected from the group consisting of SEQ ID NOs:
- 1, 2, 3, 4, or 16.
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A construct, comprising (i) the sequence of either of SEQ ID NOs. 1 or 2 operably linked to a polynucleotide comprising the sequence of any one of SEQ ID NOs 5-8, (ii) the sequence of SEQ ID NO:
- 3 operably linked to the sequence of SEQ ID NO:
- 9, (iii) the sequence of either SEQ ID NO:
- 4 or 16 operably linked to the sequence of SEQ ID NO:
- 7, (iv) the sequence of either SEQ ID NOs:
- 1 or 2 operably linked to a polynucleotide that encodes a polypeptide comprising the amino acid sequence depicted in any one of SEQ ID NOs. 9-12, or (v) the sequence depicted in either SEQ ID NO:
- 22 or SEQ ID NO:
- 23.
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A hybrid progeny plant transformed with a construct comprising the sequence of any of SEQ ID NOs. 18-26.
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Dependencies are inferred from the claim text.