Novel ways of assessing metabolic processes
AssigneeLipomics Technologies, Inc.
InventorSteven Watkins
Life of this patent
Estimating the term…
Abstract
The present invention provides methods for assessing contribution of one or more pathways to the biosynthesis of a metabolite, e.g., a lipid using the levels of the product made and the metabolite precursors for each pathway.
Claims20
A method for determining the contribution of a pathway to the biosynthesis of a lipid class comprising
- determining the level of a marker composition in a precursor,
- determining the level of the marker composition in a lipid class,
- wherein the precursor is transformed to the lipid class via a pathway, and wherein the level of the marker composition in the precursor relative to the level of the marker composition in the lipid class is indicative of the contribution of the pathway to the biosynthesis of the lipid class.
A method of determining the contribution of a first pathway and a second pathway to the biosynthesis of a lipid class comprising
- determining P1, wherein P1 is the level of a marker composition in a first precursor of a lipid class,
- wherein the first precursor is transformed to the lipid class via the first pathway,
- determining P2, wherein P2 is the level of the marker composition in a second precursor
- of the lipid class,
- wherein the second precursor is transformed to the lipid class via the second pathway,
- determining TL, wherein TL is the level of the marker composition in the lipid class,
- wherein the contribution of the first pathway is represented by (TL−P2)/(P1−P2) and the contribution of the second pathway is represented by (TL−P1)/(P2−P1).
A method of determining the level of a marker composition of phosphatidylcholine in liver comprising determining the level of the marker composition of phosphatidylcholine in plasma.
Dependencies are inferred from the claim text.