Constitutive expression cassettes for regulation of plant expression
AssigneeSunGene GmbH & Co. KGaA
InventorsUlrich Keetman, Ute Linemann, Karin Herbers, Helke Hillebrand
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Abstract
The present invention relates to expression cassettes comprising transcription regulating sequences with constitutive expression profiles in plants obtainable from Arabidopsis thaliana genes At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, At1g64090, At5g60690, At1g76350, At1g76580, At1g31930, At5g18230, At1g20970, or At4g35620.
Claims12
An expression cassette for regulating constitutive expression in plants comprising
- i) at least one transcription regulating nucleotide sequence of a plant gene, said plant gene selected from the group of genes described by the GenBank Arabidopsis thaliana genome loci At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, At1g64090, At5g60690, At1g76350, At1g76580, At1g31930, At5g18230, At1g20970, and At4g35620, or a functional equivalent thereof, and functionally linked thereto
- ii) at least one nucleic acid sequence which is heterologous in relation to said transcription regulating nucleotide sequence.
A method for identifying and/or isolating a sequence with constitutive transcription regulating activity characterized that said identification and/or isolation utilizes a nucleic acid sequence encoding a amino acid sequence as described by SEQ ID NO:
- 8, 16, 26, 32, 36, 41, 45, 50, 54, 59, 64, 68, 72, 78, 84, 88, 94, or 98 or a part of at least 15 bases thereof.
A method for providing a transgenic expression cassette for constitutive expression comprising the steps of:
- I. isolating of a constitutive transcription regulating nucleotide sequence utilizing at least one nucleic acid sequence or a part thereof, wherein said sequence is encoding a polypeptide described by SEQ ID NO:
- 8, 16, 26, 32, 36, 41, 45, 50, 54, 59, 64, 68, 72, 78, 84, 88, 94, or 98, or a part of at least 15 bases thereof, and
- II. functionally linking said constitutive transcription regulating nucleotide sequence to another nucleotide sequence of interest, which is heterolog in relation to said constitutive transcription regulating nucleotide sequence.
Dependencies are inferred from the claim text.