Dedifferentiated, programmable stem cells of monocytic origin, and their production and use
AssigneeBlasticon Biotechnologische Forschung GmbH
InventorsBernd Kremer, Fred Fandrich, Maren Ruhnke
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Abstract
The invention relates to the production of adult dedifferentiated, programmable stem cells from human monocytes by cultivation of monocytes in a culture medium which contains M-CSF and IL-3. The invention further relates to pharmaceutical preparations, which contain the dedifferentiated, programmable stem cells and the use of these stem cells for the production of target cells and target tissue.
Claims20
1-34. (canceled)
A process for the production of dedifferentiated, programmable stem cells of human monocytic origin, comprising:
- a) isolating monocytes from human blood;
- b) propagating said monocytes in a culture medium comprising cellular growth factor M-CSF;
- and
- c) cultivating said monocytes simultaneously with or subsequently to step b) in a culture medium comprising IL-3.
A process for the production of dedifferentiated, programmable stem cells of human monocytic origin in a pharmaceutical preparation, comprising:
- a) isolating monocytes from human blood;
- b) propagating said monocytes in a culture medium comprising cellular growth factor M-CSF;
- c) cultivating said monocytes simultaneously with or subsequently to step b) in a culture medium comprising IL-3;
- d) obtaining dedifferentiated, programmable stem cell of human monocytic origin from said culture medium comprising IL-3;
- and
- e) formulating a pharmaceutical preparation in a suitable medium, wherein said pharmaceutical preparation is derived from said human adult dedifferentiated programmable stem cell.
A process for preparing a dedifferentiated, programmable stem cell of human monocytic origin for introduction into a body, comprising:
- a) isolating monocytes from human blood;
- b) propagating said monocytes in a culture medium comprising cellular growth factor M-CSF;
- c) cultivating said monocytes simultaneously with or subsequently to step b) in a culture medium comprising IL-3;
- d) obtaining dedifferentiated, programmable stem cells of human monocytic origin from said culture medium comprising IL-3;
- and
- e) applying said dedifferentiated, programmable stem cell of human monocytic origin to a material.
Dependencies are inferred from the claim text.