Method to reduce contamination when culturing mycobacteria
AssigneeThe Trustees of the University of Pennsylvania
InventorRobert Whitlock
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Abstract
Provided is a method for culturing Mycobacteria in the presence of a class A lantibiotic, thereby inhibiting or reducing bacterial contamination in the culture, without significantly disadvantageously affecting or retarding mycobacterial growth. Further provided are the Mycobacteria culture produced thereby, methods for detecting Mycobacteria in a biological sample under conditions that reduce bacterial contamination, and for diagnosing a mycobacterial infection in a biological sample from a subject suspected of having such an infection, as well as, kits useful in practicing such methods.
Claims28
A method of inhibiting bacterial contamination in a Mycobacteria culture, the method comprising culturing the Mycobacteria in a medium comprising a class A lantibiotic in an amount sufficient to inhibit bacterial contamination in the Mycobacteria culture.
A method of detecting Mycobacteria in a sample, the method comprising:
- culturing a sample in a medium comprising a class A lantibiotic in an amount sufficient to inhibit bacterial contamination, and
- detecting the presence of Mycobacteria.
A method of diagnosing a mycobacterial infection in a subject, the method comprising:
- culturing a biological sample from the subject suspected of having the mycobacterial infection in a medium comprising a class A lantibiotic in an amount sufficient to inhibit bacterial contamination, and
- assessing the presence or absence of Mycobacteria in the culture, wherein the presence of Mycobacteria indicates a mycobacterial infection in the subject.
A kit for detecting or culturing Mycobacteria in a sample, the kit comprising:
- medium suitable for culturing Mycobacteria, wherein the medium comprises a class A lantiobiotic in an amount sufficient to inhibit bacterial contamination, and
- an instructional material for the use thereof.
Dependencies are inferred from the claim text.