Delivery of polynucleotides
OwnerMaya Dajee, Rolf Ehrhardt +4inventors
InventorsMaya Dajee, Rolf Ehrhardt, Hans Hofland, Leslie Mcevoy, Tony Muchamuel, Brian Schryver
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Abstract
The present invention concerns methods and formulations for non-parental delivery of nucleic acid molecules to cells. In particular, the present invention relates to methods and formulations that enhance the transport of poly- and oligonucleotides across biological membranes.
Claims76
A method for delivering a polynucleotide to a cell, said method comprising contacting a biological membrane with a formulation containing said polynucleotide, at least one penetration enhancer in a total concentration of about 0.3% to about 10% by weight, and an alcohol in a concentration of about 1% to about 60% by weight.
A method for delivering a polynucleotide to a cell, said method comprising contacting a biological membrane with an emulsion-based formulation containing said polynucleotide, at least one penetration enhancer in a total concentration of about 0.2% to about 10% by weight, and water wherein said penetration enhancer is selected from the group consisting of sodium laureth sulfate, N-lauroylsarcosine, sorbitan monolaurate 20 (Span 20) and isopropyl myristate.
A formulation comprising an oligonucleotide, at least one penetration enhancer in a total concentration of about 0.2% to about 10% by weight, and an alcohol in a concentration of about 1% to about 60% by weight.
Dependencies are inferred from the claim text.