Back to results
US 2006/0068496 A1Published application
Differentiation of stem cells
OwnerJames Kellyinventor
Life of this patent
Estimating the term…
Abstract
Disclosed are compositions and methods for identifying specific cell types.
Claims75
A pluripotent stem cell containing a nucleic acid segment, wherein the nucleic acid segment comprises the structure P-I, wherein P is a transcriptional control element and I is a sequence encoding a marker, wherein the marker comprises a transformation agent.
A method of deriving stem cell derived conditionally immortal cell types, the method comprising:
- transfecting stem cells with a nucleic acid segment comprising the structure P-I, wherein P is a transcriptional control element and I is a sequence encoding a marker, wherein the marker comprises a transformation agent;
- culturing the stem cells under conditions in which the transcriptional control element is activated, whereby I is preferentially or selectively expressed, thereby deriving stem cell derived conditionally immortal cell types.
A method of deriving differentiated cells from stem cells, the method comprising:
- transfecting stem cells with a nucleic acid segment comprising the structure P-I, wherein P is a transcriptional control element and I is a sequence encoding a marker, wherein the marker comprises a transformation agent;
- culturing the stem cells under conditions in which the transcriptional control element is activated, whereby I is preferentially or selectively expressed, thereby deriving differentiated cells.
A method of deriving differentiated cells from stem cells, the method comprising:
- transfecting stem cells with a nucleic acid segment comprising the structure P-I, wherein P is a transcriptional control element and I is a sequence encoding a marker;
- culturing the stem cells under conditions in which the transcriptional control element is activated, whereby I is preferentially or selectively expressed, wherein the conditions in which the transcriptional control element is activated are conditions in which the stem cells differentiate thereby deriving differentiated cells.
Dependencies are inferred from the claim text.