Compositions to ameliorate protein misfolding and aggregation

An isolated polynucleotide, in association with a viral expression vector

• wherein the polynucleotide encodes a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:8, or a fragment or a conservative substitution thereof,
• and wherein the polypeptide encoded by the sequence as set forth in SEQ ID NO:8 prevents protein misfolding and aggregation.

An isolated polynucleotide, comprising a nucleic acid sequence that hybridizes to a polynucleotide comprising a sequence as set forth in SEQ ID NO:7,

• wherein the isolated polynucleotide is in association with a viral vector, and
• wherein the isolated polynucleotide encodes a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:8 or conservative substitutions thereof,
• and wherein said polypeptide prevents protein misfolding and aggregation.

A viral expression vector, comprising a nucleic acid sequence that hybridizes to a polynucleotide comprising a sequence as set forth in SEQ ID NO:

• 7 that encodes a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:
• 8, or a fragment or a conservative substitution thereof,
• wherein the polypeptide prevents protein misfolding and aggregation.

A method for producing a polynucleotide encoding a polypeptide that prevents protein misfolding and aggregation, comprising contacting a polynucleotide sample with a polynucleotide comprising at least 15 consecutive nucleotides of SEQ ID NO:7, or at least 15 consecutive nucleotides of a complement thereof.

A pharmaceutical composition for treating disorders associated with protein aggregation comprising an effective amount of a polynucleotide sequence hybridizing to a polynucleotide comprising a sequence as set forth in SEQ ID NO:7, wherein the polynucleotide is associated with a viral vector and at least one physiologically-acceptable carrier,

• and wherein the polynucleotide sequence expresses a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:8 or a fragment or a conservative substitution thereof, wherein the polypeptide prevents protein misfolding or aggregation.