Purification of biomolecules from contaminating intact nucleic acids
AssigneeSigma-Aldrich Co.
InventorsBrian Ward, Brian Buntaine
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Abstract
The present invention provides methods for the removal, destruction or inactivation of intact nucleic acids contaminating desired biomolecules by the use of small molecule nucleic acid cleavage agents.
Claims38
A method for the isolation of a biomolecule originating from natural, genetically engineered or biotechnological biological sources, which is substantially free of intact nucleic acids, comprising the following steps:
- a. treatment of a medium comprising the biomolecule to be isolated with a small molecule nucleic acid cleavage agent;
- and
- b. purification of the resultant medium comprising the biomolecule to remove cleaved DNA and/or the small molecule nucleic acid cleavage agent.
A kit that is suitable for use in the isolation of a biomolecule originating from natural, genetically engineered or biotechnological biological sources, which is substantially free from intact nucleic acids, comprising the following steps:
- a. treatment of a medium comprising the biomolecule to be isolated with a solution comprising a small molecule nucleic acid cleavage agent;
- and
- b. purification of the resultant medium comprising the biomolecule to remove damaged DNA and/or the small molecule nucleic acid cleavage agent;
- wherein the kit comprises a small molecule nucleic acid cleavage agent, chromatographic matrices for purification of a biomolecule and aqueous buffer solutions.
Dependencies are inferred from the claim text.