Universal and target specific reagent beads for nucleic acid amplification
AssigneeCepheid
InventorWilliam McMillan
Life of this patent
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Abstract
The present invention relates to methods and compositions providing optimized reaction conditions for nucleic acid amplification reactions and for methods and compositions that reduce contamination of amplification reactions during set up.
Claims29
A multiple bead assay system for nucleic acid amplification with an internal control oligonucleotide, the multiple bead assay system comprising a set of reagent beads, wherein the set of reagent beads comprises:
- a) a first lyophilized reagent bead comprising:
- (i) at least one enzyme for nucleic acid amplification;
- and
- (ii) nucleotides, buffers, salts, or cofactors;
- and
- b) a second lyophilized reagent bead comprising:
- i) oligonucleotide primers for specific amplification of the control oligonucleotide;
- and
- ii) primers for specific amplification of at least one analyte nucleic acid sequence.
A multiple bead assay system for nucleic acid amplification, the multiple bead assay system comprising a set of reagent beads, wherein the set of reagent beads comprises:
- a) a first lyophilized reagent bead comprising:
- (i) at least one enzyme for nucleic acid amplification;
- and
- (ii) nucleotides, buffers, salts, or cofactors;
- and
- b) a second lyophilized reagent bead comprising oligonucleotide primers for amplification of at least one analyte nucleic acid sequence.
A method for performing a nucleic acid amplification assay with an internal control oligonucleotide, the method comprising the steps of:
- a) combining in aqueous solution:
- i) a first lyophilized reagent bead that comprises at least one enzyme for nucleic acid amplification, wherein the first lyophilized reagent bead further comprises nucleotides, buffers, salts, or cofactors;
- and
- ii) a second lyophilized reagent bead that comprises oligonucleotide primers for specific amplification of the control oligonucleotide, wherein the second lyophilized reagent bead further comprises primers for specific amplification of at least one analyte nucleic acid sequence, if the analyte nucleic acid sequence is present in the solution;
- and
- b) performing the nucleic acid amplification assay to amplify the internal control oligonucleotide and the at least one analyte nucleic acid sequence, if present.
Dependencies are inferred from the claim text.