Cell motility assay
OwnerLakshmi Kamathinventor
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Abstract
An electrooptical method is disclosed for quantifying the motility response of cells to external stimuli, wherein a target cell population (formed as a function of said response) is subsequently optically differentiated by the addition of an optical differentiation solution, and whereby the targeted cell population becomes more or less detectable to an electrooptical reading device. The method can be used to perform chemotactic assays. For such and other purposes, the method can be performed utilizing high throughput robotic automation.
Claims5
An aqueous masking solution consisting essentially of approximately 1% carbon black and 50% glycerol.
A chemotactic assay kit suitable for analyzing cells, said kit comprising enclosed within a common package:
- a multi-well array comprising nested or nestable pairs of fluid receptacles, each pair when nested having and upstream zone and a downstream zone separated by a translucent isoporous membrane;
- and
- an aqueous solution comprising carbon black and glycerol.
Dependencies are inferred from the claim text.