Scoring estrogen and progesterone receptors expression based on image analysis

An method of scoring ER and PR from image data obtained from histological slides, the method including the steps of:

• a) determining the number of pixels in an image having relatively dark intensities compared to other pixels in an image,
• b) determining pixel number thresholds to quantify scoring, and
• c) comparing the number of relatively dark pixels with the thresholds and scoring ER or PR in accordance therewith.

An method of scoring ER and PR from image data obtained from histological slides, the method including the steps of:

• a) determining what proportion of total blob area is brown blob area in an image,
• b) determining brown blob area proportion thresholds to quantify scoring, and
• c) comparing the brown blob area proportion with the thresholds and scoring ER or PR in accordance therewith.

Computer apparatus for scoring ER and PR from image data obtained from histological slides, the apparatus being programmed to determine the number of relatively dark image pixels compared to relatively bright image pixels and to score ER or PR in accordance with the magnitude of the number of relatively dark pixels.

Computer apparatus for scoring ER and PR from image data obtained from histological slides, the apparatus being programmed to:

• a) determine the number of pixels in an image having relatively dark intensities compared to other pixels in an image,
• b) determine pixel number thresholds to quantify scoring, and
• c) compare the number of relatively dark pixels with the thresholds and scoring ER or PR in accordance therewith.

Computer apparatus for scoring ER and PR from image data obtained from histological slides, the apparatus being programmed to:

• a) determine what proportion of total blob area is brown blob area in an image,
• b) determine brown blob area proportion thresholds to quantify scoring, and
• c) compare the brown blob area proportion with the thresholds and scoring ER or PR in accordance therewith.

A computer program for scoring ER and PR from image data obtained from histological slides, the program including instructions for controlling computer apparatus to determine the number of relatively dark image pixels compared to relatively bright image pixels and scoring ER or PR in accordance with the magnitude of the number of relatively dark pixels.

A computer program for scoring ER and PR from image data obtained from histological slides, the program including instructions for controlling computer apparatus to:

• a) determine the number of pixels in an image having relatively dark intensities compared to other pixels in an image,
• b) determine pixel number thresholds to quantify scoring, and
• c) compare the number of relatively dark pixels with the thresholds and scoring ER or PR in accordance therewith.

A computer program for scoring ER and PR from image data obtained from histological slides, the program including instructions for controlling computer apparatus to:

• a) determine what proportion of total blob area is brown blob area in an image,
• b) determine brown blob area proportion thresholds to quantify scoring, and
• c) compare the brown blob area proportion with the thresholds and scoring ER or PR in accordance therewith.