Data frame selection for cytometer analysis
AssigneeHoneywell International Inc.
InventorsBernard Fritz, Aravind Padmanabhan, Peter Reutiman
Life of this patent
Estimating the term…
Abstract
A system for detecting when a core stream in a channel of a flow device is adequate for sufficient data accession. The system may determine the time period from the beginning of a sample run in a channel of a flow device to when the core stream is adequate. Data of detected FALS versus SALS light scattered by particles of the core stream may be plotted in data frames during a series of time intervals. The quality of the grouping and count rate of the data may indicate which frame and corresponding time interval reveal an adequate core stream.
Claims42
A method for detecting an optimum core stream in a channel, comprising:
- at a starting time, providing a sample of particles to form a core stream that flows in a channel;
- directing light at the particles of the core stream flowing in the channel;
- detecting light scattered by the particles in the core stream during a series of time intervals;
- converting the light scattered by the particles detected at a forward angle light scattering (FALS) angle and a small angle light scattering (SALS) angle, into FALS versus SALS data for each interval of time of the series of time intervals after the starting time;
- analyzing the FALS versus SALS data for each interval of time;
- and
- selecting a time duration from the series of time intervals where the FALS versus SALS data exhibit an approximately certain count and grouping of data.
An optimization detection system comprising:
- a processor connected to a FALS and SALS detector mechanism situated proximate to a light source arrangement having a flow channel situated between the FALS and SALS detector mechanism and the light source arrangement;
- and
- wherein the processor is for recording signals from the FALS and SALS detector mechanism.
A method for detecting an optimum core stream in a flow channel, comprising:
- beginning an insertion of particles to form a core stream sheathed by a fluid to flow in a channel;
- directing light at the particles in the channel;
- detecting and converting light scattered by the particles into electrical signals;
- and
- processing the electrical signals in accordance with an algorithm for a determination of when the core stream is optimum for data taking.
Means for detecting an optimum core stream in a channel of a flow device, comprising:
- means for providing particles to form a core stream that flows in a channel starting at time zero;
- means for directing light at the particles of the core stream flowing in the channel;
- and
- means for determining an optimum core stream from light scattered by the particles.
A data frame selection system comprising:
- a processor connected to a light scatter detector mechanism proximate to a light source arrangement having a flow channel situated between the light source arrangement and the light scatter detector mechanism;
- and
- wherein the processor comprises an analyzer for identifying an optimum flow of a core stream in the flow channel sufficient for data taking.
Dependencies are inferred from the claim text.