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AssigneeMichigan State University
InventorsXiaoming Bao, John Ohlrogge, Michael Pollard
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The present invention provides cyclopropane fatty acid synthase genes and proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also provides methods of using cyclopropane fatty acid synthase genes and proteins, including in their expression in transgenic organisms and in the production of cyclopropane fatty acids in plant oils, and in particular seed oils.
A composition comprising an isolated nucleic acid sequence encoding a plant cyclopropane fatty acid synthase, wherein said sequence is at least 90% identical to a sequence selected from the group consisting of SEQ ID NO 1, 3, 4, 5, and 6 and wherein said sequence encodes a protein having cyclopropane fatty acid synthase activity.
A composition comprising a nucleic acid sequence encoding a plant cyclopropane fatty acid synthase, wherein said sequence is at least 90% identical to a sequence selected from the group consisting of SEQ ID NO 1, 3, 4, 5, and 6 and wherein said sequence encodes a protein having cyclopropane fatty acid synthase activity operably linked to a heterologous promoter.
A composition comprising a vector comprising a nucleic acid sequence encoding a plant cyclopropane fatty acid synthase, wherein said sequence is at least 90% identical to a sequence selected from the group consisting of SEQ ID NO 1, 3, 4, 5, and 6 and wherein said sequence encodes a protein having cyclopropane fatty acid synthase activity.
A composition comprising a purified polypeptide encoded by a nucleic acid sequence of encoding a plant cyclopropane fatty acid synthase, wherein said sequence is at least 90% identical to a sequence selected from the group consisting of SEQ ID NO 1, 3, 4, 5, and 6 and wherein said sequence encodes a protein having cyclopropane fatty acid synthase activity.
A composition comprising a purified plant cyclopropane fatty acid synthase, wherein said cyclopropane fatty acid synthase comprises an amino acid sequence selected from the group consisting of SEQ ID NO:2, 7, 8, 9, 10, and 11 and sequences at least 90% identical thereto.
A composition comprising an isolated nucleic acid sequence encoding a protein comprising an amino acid sequence homologous to an amino terminus of a plant cyclopropane fatty acid synthase.
A composition comprising a purified protein comprising an amino acid sequence homologous to an amino terminus of a plant cyclopropane fatty acid synthase.
A method for expressing a plant cyclopropane fatty acid synthase in a plant, comprising;
A method for decreasing expression of CPA-FAS in plants, comprising:
A method for decreasing expression of CPA-FAS in plants, comprising:
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