Feline infectious peritonitis (FIP) and systemic multi-organ coronavirus biomarkers and screening methods
OwnerKimberly Austin, Sanjay Kapil +1inventors
InventorsKimberly Austin, Sanjay Kapil, Jeong-Ki Kim
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Abstract
Methods for screening for FIP infection or other multi-organ coronaviruses are disclosed, as well as isolated antibodies and kits useful for performing such methods. Biomarkers for multi-organ coronavirus infections include soluble enolase; antibodies to enolase; and circulating immune complexes that contain enolase. The methods find application in diagnosis, treatment, vaccine-development, and selection or breeding for disease-resistance.
Claims25
A method for screening an individual of the Felidae family for FIP CoV exposure or infection, said method comprising:
- a) determining whether or not a sample comprising circulating immune complexes from said individual comprises enolase.
A method for screening an individual of the Felidae family for FIP CoV exposure or infection, said method comprising:
- a) determining whether or not a sample from said individual comprises an antibody specific for enolase.
A method for determining whether or not a test vaccine for a multi-organ CoV is safe for administration comprising:
- (a) administering said test vaccine to an individual;
- (b) determining whether or not an elevated level of antibodies specific for enolase is produced in said individual relative to a control individual not administered said test vaccine, wherein an elevated level of antibodies specific for enolase is indicative that said test vaccine is not safe for administration.
An isolated antibody specific for Felidae enolase, wherein said isolated antibody is not specific for human enolase.
An article of manufacture comprising an isolated antibody specific for Felidae enolase, wherein said isolated antibody is not specific for human enolase.
A method for evaluating if a test FIP vaccine has an increased tendency to induce an ADE response in a individual of the Felidae family, said method comprising:
- (a) administering said test FIP vaccine to an individual of the Felidae family;
- (b) determining whether or not, after said test FIP vaccine administration, said individual exhibits CICs comprising enolase in an elevated amount relative to a control individual not administered said test vaccine, wherein said elevated production of CICs is indicative that said test FIP vaccine has an increased tendency to induce an ADE response.
An isolated polynucleotide comprising a nucleic acid having 91% or higher sequence identity to SEQ ID NO:9.
An isolated polynucleotide comprising a nucleic acid encoding a polypeptide having 97% or higher sequence identity to the amino acid sequence set forth in SEQ ID NO:10.
An isolated polypeptide comprising an amino acid sequence having 97% or higher sequence identity to SEQ ID NO:10.
Dependencies are inferred from the claim text.