Compositions for the delivery of therapeutic agents and uses thereof
AssigneeProtiva Biotherapeutics, Inc.
InventorsIan MacLachlan, Lloyd Jeffs
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Abstract
The present invention provides drug delivery vehicles comprising polytheylyene-lipid conjugates (PEG-lipid), wherein the circulation lifetime and biodistribution of the drug delivery vehicles are regulated by the PEG-lipid. More particularly, the present invention provides liposomes, SNALP and SPLP comprising such PEG-lipid conjugates, and methods of using such compositions to selectively target a tumor site or other tissue of interest (e.g., liver, lung, spleen, etc.).
Claims48
A method of introducing a nucleic acid into a tumor cell, said method comprising contacting said tumor cell with a nucleic acid-lipid particle comprising a cationic lipid, a noncationic lipid, a PEG-lipid conjugate, and a nucleic acid, wherein the alkyl or acyl chains of the lipid portion of said PEG-lipid conjugate comprise from 12 to 20 carbon atoms.
A method of introducing a nucleic acid to the lung of a mammal, said method comprising administering to said mammal a nucleic acid-lipid particle comprising a cationic lipid, a noncationic lipid, a PEG-lipid conjugate, and a nucleic acid, wherein the alkyl or acyl chains of the lipid portion of said PEG-lipid conjugate comprise from 16 to 20 carbon atoms.
A method of introducing a nucleic acid to the liver of a mammal, said method comprising administering to said mammal a nucleic acid-lipid particle comprising a cationic lipid, a noncationic lipid, a PEG-lipid conjugate, and a nucleic acid, wherein the alkyl or acyl chains of the lipid portion of said PEG-lipid conjugate comprise from 8 to 14 carbon atoms.
A method of introducing a nucleic acid to the spleen of a mammal, said method comprising administering to said mammal a nucleic acid-lipid particle comprising a cationic lipid, a noncationic lipid, a PEG-lipid conjugate, and a nucleic acid, wherein the alkyl or acyl chains of the lipid portion of said PEG-lipid conjugate comprise from 8 to 14 carbon atoms.
Dependencies are inferred from the claim text.