Lutzomyia longipalpis polypeptides and methods of use
AssigneesThe United States of America, as represented by the Secretary, Department of Health and Human Services, Fundação Oswaldo Cruz (FIOCRUZ)
InventorsJesus Valenzuela, Jose Ribeiro, Aldina Barral, Manoel Netto, Claudia Brodskyn, Regis Gomes
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Abstract
Substantially purified salivary Lu. longipalpis polypeptides, and polynucleotides encoding these polypeptides are disclosed. Vectors and host cells including the Lu. longipalpis polynucleotides are also disclosed. In one embodiment, a method is disclosed for inducing an immune response to sand fly saliva. In other embodiments, methods for treating, diagnosing, or preventing Leishmaniasis are disclosed.
Claims38
A substantially purified salivary Lu. longipalpis polypeptide.
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A method of diagnosing Leishmania infection in a subject, comprising:
- contacting a solid substrate comprising at least three, six, or ten polypeptides, wherein each of the polypeptides comprises one amino acid sequence as set forth as SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 17, SEQ ID NO:
- 19, SEQ ID NO:
- 21, SEQ ID NO:
- 25, SEQ ID NO:
- 27, SEQ ID NO:
- 29, SEQ ID NO:
- 31, SEQ ID NO:
- 33, SEQ ID NO:
- 35, SEQ ID NO:
- 41, SEQ ID NO:
- 43, SEQ ID NO:
- 45, SEQ ID NO:
- 47, SEQ ID NO:
- 49, SEQ ID NO:
- 51, SEQ ID NO:
- 53, SEQ ID NO:
- 55, SEQ ID NO:
- 59, SEQ ID NO:
- 61, SEQ ID NO:
- 63, SEQ ID NO:
- 65, or SEQ ID NO:
- 67, or an immunogenic fragment thereof;
- contacting the solid substrate with a sample obtained from the subject;
- and
- detecting binding of a component of the sample to at least one polypeptide on the solid substrate, wherein detection of binding of the component to the substrate indicates that the subject is infected with Leishmania,
- thereby diagnosing Leishmania infection in the subject.
Dependencies are inferred from the claim text.