Control of plant growth and developmental process
AssigneesUniversiteit Leiden, Stichting Binair Vector Systeem
InventorsPaul Hooykaas, Engelbertus van der Zaal
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Abstract
The invention relates to a method of modulating plant growth or developmental processes in a plant or plant cell, more particularly, organogenesis and/or embryogenesis, through the provision of a plant protein which can act as a transcriptional regulator of the processes. The invention further provides a plant and propagating material thereof which contains, in its genome, a nucleotide sequence encoding a protein of the invention, the protein characterized in that it includes the amino acid sequence PRGRPPGSKNK (SEQ ID NO:2).
Claims29
A method of modulating plant growth or developmental processes in a plant or plant cell, said method comprising:
- introducing into said plant or plant cell a protein that has at least 200 amino acids, wherein the amino acid sequence PRGRPPGSKNK (SEQ ID NO:2) is present in the N-terminal part, and wherein a DUF296 domain having a cysteine to serine substitution is separated from said amino acid sequence by about 13-20 amino acid residues,
- thus modulating growth or developmental processes in said plant or plant cell.
A method for producing asexually derived embryos, said method comprising:
- transforming a plant cell with a nucleotide sequence encoding a protein that has at least 200 amino acids, wherein the amino acid sequence PRGRPPGSKNK (SEQ ID NO:2) is present in the N-terminal part, and wherein a DUF296 domain having a cysteine to serine substitution is separated from said amino acid sequence by about 13-20 amino acid residues;
- growing said plant cell to produce tissue;
- selecting said tissue for the presence of said nucleotide sequence;
- assaying said tissue for asexual embryo production;
- and
- maintaining or growing isolated mature or immature embryos in culture.
A method for producing asexually derived embryos, said method comprising:
- introducing into said plant cell a protein that has at least 200 amino acids, wherein the amino acid sequence PRGRPPGSKNK (SEQ ID NO:2) is present in the N-terminal part, and wherein a DUF296 domain having a cysteine to serine substitution is separated from said amino acid sequence by about 13-20 amino acid residues, to produce a modified plant cell;
- growing said modified plant cell to produce tissue;
- assaying said tissue for asexual embryo formation;
- and
- maintaining or growing isolated mature or immature embryos in culture.
A method for producing an apomictic plant, said method comprising:
- transforming a plant with a nucleotide sequence encoding a protein that has at least 200 amino acids, wherein the amino acid sequence PRGRPPGSKNK (SEQ ID NO:2) is present in the N-terminal part, and wherein a DUF296 domain having a cysteine to serine substitution is separated from said amino acid sequence by about 13-20 amino acid residues, to produce a transformed plant;
- or introducing said protein into said plant;
- selecting said transformed plant for the presence of said nucleotide sequence;
- assaying said transformed plant for asexual embryo production;
- maintaining or growing isolated mature or immature embryos in culture;
- and
- production of complete plants from the mature embryos.
A method for enhancing the regenerative capacity of a plant, said method comprising:
- transforming a plant cell with a nucleotide sequence encoding a protein that has at least 200 amino acids, wherein the amino acid sequence PRGRPPGSKNK (SEQ ID NO:2) is present in the N-terminal part, and wherein a DUF296 domain having a cysteine to serine substitution is separated from said amino acid sequence by about 13-20 amino acid residues, or introducing said protein into said plant cell to produce a modified plant cell;
- growing said modified plant cell to produce tissue;
- assaying said tissue for enhanced regeneration as compared to wild-type tissue;
- and
- regeneration of complete plants from said tissue.
A method of selecting a modified plant, said method comprising:
- transforming a normally non-regenerative plant with a nucleotide sequence encoding a protein that has at least 200 amino acids, wherein the amino acid sequence PRGRPPGSKNK (SEQ ID NO:2) is present in the N-terminal part, and wherein a DUF296 domain having a cysteine to serine substitution is separated from said sequence by about 13-20 amino acid residues;
- or introducing said protein into said plant, to produce said modified plant;
- and
- determining whether said modified plant is able to regenerate under conditions in which said normally non-regenerative plant does not regenerate.
A method of producing a protein of interest, said method comprising:
- preparing a first nucleotide sequence encoding a protein involved in transcriptional regulation characterized in that it has at least 200 amino acids, wherein the amino acid sequence PRGRPPGSKNK (SEQ ID NO:2) is present in the N-terminal part, and wherein a DUF296 domain is separated from said amino acid sequence by about 13-20 amino acid residues;
- preparing a second nucleotide sequence encoding a protein of interest under the control of a regulatory element, wherein said regulatory element is bound by the expression product of said first nucleotide sequence which induces the expression of said protein of interest;
- transforming a plant with the first and second nucleotide sequence;
- selecting said transformed plant for occurrence of said first and second nucleotide sequence;
- and
- growing said transformed plant in order to produce said protein of interest.
A plant protein having at least 200 amino acids, wherein the amino acid sequence PRGRPPGSKNK is present in the N-terminal part, and wherein a DUF296 domain having a cysteine to serine substitution is separated from said sequence by about 13-20 amino acid residues wherein said plant protein has Genbank accession no. NP—177776.1, or a functional part thereof.
Dependencies are inferred from the claim text.