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US 2006/0041955 A1Published application
Marker mapping and resistance gene associations in soybean
AssigneesPioneer Hi-Bred International, Inc., Virginia Tech Intellectual Properties, Inc.
InventorsMichael Godwin, Feng Han, Alec Hayes, Xu Hu, Soon-Chun Jeong, Guihua Lu, M. A. Maroof
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Abstract
The invention provides novel molecular genetic markers in soybean, where the markers are useful, for example, in the marker-assisted selection of gene alleles that impart disease-resistance, thereby allowing the identification and selection of a disease-resistant plant. The markers also find use in positional cloning of disease-resistance genes.
Claims35
A method for selecting a disease-resistant soybean plant, the method comprising:
- a) detecting a marker nucleic acid, wherein the marker nucleic acid maps ten centimorgans or less from a disease-resistance gene allele, wherein the marker comprises a nucleotide sequence corresponding to an expressed gene sequence, and wherein the disease-resistance gene allele is selected from resistance alleles of soybean mosaic virus resistance gene 4 (Rsv4), frogeye leaf spot resistance gene (Rcs3), brown stem rot resistance gene 1 (Rbs1), brown stem rot resistance gene 2 (Rbs2) and brown stem rot resistance gene 3 (Rbs3);
- and,
- b) selecting a plant comprising the marker nucleic acid, thereby selecting a disease-resistant plant.
A method for positionally cloning a soybean genomic nucleic acid comprising a disease-resistance gene allele, the method comprising:
- a) identifying a soybean genomic nucleic acid comprising the disease-resistance gene allele and a marker nucleic acid, the marker nucleic acid mapping ten centimorgans or less from the disease-resistance gene allele, the marker nucleic acid comprising a nucleotide sequence corresponding to an expressed gene sequence, wherein the disease-resistance gene allele is selected from resistance alleles of soybean mosaic virus resistance gene 4 (Rsv4), frogeye leaf spot resistance gene (Rcs3), brown stem rot resistance gene 1 (Rbs1), brown stem rot resistance gene 2 (Rbs2) and brown stem rot resistance gene 3 (Rbs3);
- and,
- b) isolating the genomic nucleic acid comprising the marker nucleic acid, thereby positionally cloning the disease-resistance gene allele.
A composition comprising a nucleic acid, the nucleic acid selected from:
- a) an amplification primer that is capable of initiating DNA polymerization by a DNA polymerase on a soybean nucleic acid template to generate a single-stranded amplicon, wherein the amplicon comprises:
- i) the nucleotide sequence of SEQ ID NO:
- 1, wherein the amplification primer is complementary or partially complementary to the first 29 nucleotides of SEQ ID NO:
- 1;
- ii) the nucleotide sequence of SEQ ID NO:
- 2;
- iii) a portion of at least 20 contiguous nucleotides of (i) or (ii);
- or
- iv) a complementary nucleic acid of (i), (ii) or (iii);
- and,
- b) an amplification primer pair that is capable of initiating DNA polymerization by a DNA polymerase on a soybean nucleic acid template to generate a double-stranded amplicon, wherein the amplicon comprises:
- i) the nucleotide sequence of SEQ ID NO:
- 1, wherein one member of the amplification primer pair is complementary or partially complementary to the first 29 nucleotides of SEQ ID NO:
- 1;
- ii) the nucleotide sequence of SEQ ID NO:
- 2;
- or,
- iii) a portion of at least 20 contiguous nucleotides of (i) or (ii).
15 more elements
An isolated nucleic acid comprising:
- a) a polynucleotide corresponding to SEQ ID NO:
- 1 or SEQ ID NO:
- 2;
- b) a unique subsequence of (a);
- c) a polynucleotide that hybridizes under stringent conditions to the nucleic acid of (a) that is a polynucleotide sequence other than a sequence encoded by GenBank Accession Number AB006748, AB007126, AB007127 or AF402603;
- d) a polynucleotide encoding a polypeptide comprising at least 138 contiguous amino acids of SEQ ID NO:
- 7;
- e) a polynucleotide that is at least about 70% identical to a polynucleotide sequence coresponding to SEQ ID NO:
- 2;
- f) a polynucleotide comprising a portion of at least 258 contiguous nucleotides of SEQ ID NO:
- 1;
- g) a polynucleotide comprising a portion of at least 23 contiguous nucleotides of SEQ ID NO:
- 2;
- or,
- h) a polynucleotide complementary to a polynucleotide of (a)-(g).
12 more elements
A recombinant soybean plant comprising a recombinant nucleic acid, wherein the recombinant nucleic acid comprises:
- a) a polynucleotide corresponding to SEQ ID NO:
- 1 (SSLP039);
- b) a polynucleotide corresponding to SEQ ID NO:
- 2 (SSLP090);
- c) a unique subsequence of (a) or (b);
- d) a polynucleotide that hybridizes under stringent conditions to the nucleic acid of (a) that is a polynucleotide sequence other than a sequence encoded by GenBank Accession Number AB006748, AB007126, AB007127 or AF402603;
- e) a polynucleotide that is at least 70% identical to a nucleic acid of (a) that is a polynucleotide sequence other than a sequence encoded by GenBank Accession Number AB006748, AB007126, AB007127 or AF402603;
- f) a polynucleotide that hybridizes under stringent conditions to the nucleic acid of (b)
- g) a polynucleotide that is at least 70% identical to a polynucleotide of (b)
- h) a polynucleotide complementary to a polynucleotide of (a)-(g);
- and wherein the recombinant soybean plant is more resistant to at least one of soybean mosaic virus, frogeye leaf spot and brown stem rot than an isogenic soybean plant not comprising the recombinant nucleic acid.
8 more elements
An isolated polypeptide comprising an amino acid sequence selected from:
- a) an amino acid sequence of SEQ ID NO:
- 7 (SSLP039);
- b) an amino acid sequence of SEQ 1N NO:
- 9 (SSLP090);
- c) a unique subsequence of (a) or (b);
- d) an amino acid sequence encoded by the polynucleotide of SEQ ID NO:
- 1 (SSLP039);
- e) an amino acid subsequence of at least 138 contiguous amino acids of (a) or (d);
- and,
- f) an amino acid sequence encoded by a polynucleotide that hybridizes under stringent conditions over substantially the entire length of a polynucleotide of SEQ ID NO:
- 1, wherein the polynucleotide that hybridizes over substantially the entire length of a polynucleotide of SEQ ID NO:
- 1 is a polynucleotide sequence other than a sequence encoded by GenBank Accession Number AB006748, AB007126 or AB007127.
9 more elements
Dependencies are inferred from the claim text.