P450 polynucleotides, polypeptides, and uses thereof
OwnerZhihong Cookinventor
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Abstract
Isolated P450 polynucleotides and polypeptides are disclosed, including isolated cpd polynucleotide and CPD polypeptide sequences. The polypeptides can be orthologous CPD polypeptides to Arabidopsis CPD. Recombinant vectors, host cells, transgenic plants, and seeds that include the polynucleotides and/or polypeptides are also disclosed, as well as methods for preparing and using the same.
Claims73
An isolated polynucleotide comprising a nucleic acid encoding a polypeptide having:
- (a) about 80% or greater sequence identity to the GmCPD1 amino acid sequence set forth in SEQ ID NO:8;
- (b) about 90% or greater sequence identity to each of domain A, domain B, and the heme-binding domain of GmCPD1;
- and
- (c) about 80% or greater sequence identity to domain C of GmCPD1.
A transgenic plant comprising at least one exogenous polynucleotide comprising a nucleic acid encoding a polypeptide having
- (a) about 80% or greater sequence identity to the GmCPD1 amino acid sequence set forth in SEQ ID NO:8;
- (b) about 90% or greater sequence identity to each of domain A, domain B, and the heme-binding domain of GmCPD1;
- and
- (c) about 80% or greater sequence identity to domain C of GmCPD1.
An isolated polypeptide having:
- (a) about 80% or greater sequence identity to the GmCPD1 amino acid sequence set forth in SEQ ID NO:8;
- (b) about 90% or greater sequence identity to each of domain A, domain B, and the heme-binding domain of GmCPD1;
- and
- (c) about 80% or greater sequence identity to domain C of GmCPD1.
An isolated polynucleotide comprising a nucleic acid encoding a polypeptide having about 85% or greater sequence identity to an amino acid sequence set forth in the Alignment Table.
A transgenic plant comprising at least one exogenous polynucleotide, said at least one exogenous polynucleotide comprising a nucleic acid encoding a polypeptide:
- (a) having about 85% or greater sequence identity to an amino acid sequence set forth in the Alignment Table;
- or
- (b) corresponding to the Consensus Sequence set forth in the Alignment Table.
An isolated polynucleotide comprising a nucleic acid encoding a polypeptide having about 85% or greater sequence identity to an amino acid sequence set forth in the Alignment Table, wherein said amino acid sequence is selected from the Corn CPD (SEQ ID NO:5), Rice CPD (SEQ ID NO:6), Soy1 CPD (SEQ ID NO:8), and Soy2 CPD (SEQ ID NO:7) amino acid sequences.
A method of modulating the height of a plant, said method comprising:
- a) introducing into a plant cell an exogenous nucleic acid comprising a polynucleotide sequence encoding a polypeptide having 80% or greater sequence identity to an amino acid sequence set forth in the Alignment Table, wherein a plant produced from said plant cell has a different height as compared to a corresponding control plant that does not comprise said exogenous nucleic acid, and wherein said exogenous nucleic acid further comprises a broadly expressing promoter operably linked to said polynucleotide.
A method of modulating the height of a plant, said method comprising:
- a) introducing into a plant cell an exogenous nucleic acid comprising a polynucleotide sequence encoding a polypeptide having 80% or greater sequence identity to an amino acid sequence set forth in the Alignment Table, wherein a plant produced from said plant cell has different height as compared to a corresponding control plant that does not comprise said exogenous nucleic acid, and wherein said amino acid sequence is an amino acid sequence set forth in the Alignment Table other than the Arabidopsis amino acid sequence
An isolated polypeptide having about 85% or greater sequence identity to an amino acid sequence set forth in the Alignment Table, wherein said amino acid sequence is selected from the Corn CPD (SEQ ID NO:5), Rice CPD (SEQ ID NO:6), Soy1 CPD (SEQ ID NO:8), and Soy2 CPD (SEQ ID NO:7) amino acid sequences.
A transgenic plant comprising at least one exogenous polynucleotide, said at least one exogenous polynucleotide comprising a nucleic acid encoding a polypeptide having about 85% or greater sequence identity to an amino acid sequence set forth in the Alignment Table, wherein said amino acid sequence is selected from the Corn CPD (SEQ ID NO:5), Rice CPD (SEQ ID NO:6), Soy1 CPD (SEQ ID NO:8), and Soy2 CPD (SEQ ID NO:7) amino acid sequences.
A method of modulating the height of a plant, said method comprising:
- a) introducing into a plant cell an exogenous nucleic acid comprising a polynucleotide sequence encoding a polypeptide having 80% or greater sequence identity to an amino acid sequence set forth in the Alignment Table, wherein a plant produced from said plant cell has a different height as compared to a corresponding control plant that does not comprise said exogenous nucleic acid.
Dependencies are inferred from the claim text.