Transgenic animals and uses thereof
AssigneesHematech, LLC, Kirin Beer Kabushiki Kaisha
InventorsJames Robl, Yoshimi Kuroiwa, Poothappillai Kasinathan, Isao Ishida, Kazuma Tomizuka
Life of this patent
Estimating the term…
Abstract
In general, the invention features genetically modified non-human mammals (e.g., bovines and other ungulates), and methods of making these mammals. In particular, the invention features transgenic ungulates having reduced levels of endogenous IgM heavy chain and/or prion protein.
Claims84
A transgenic ungulate whose genome comprises a mutation of two genes encoding IgM heavy chain.
A transgenic ungulate producing less than 10% of endogenous IgM heavy chain, relative to a control ungulate, and whose genome comprises a mutation of a gene encoding IgM heavy chain.
A transgenic ungulate somatic cell whose genome comprises a mutation of two genes encoding IgM heavy chain.
A transgenic bovine somatic cell whose genome comprises a hemizygous mutation of IgμAY.
A transgenic bovine somatic cell whose genome comprises a homozygous mutation of IgμAY.
A bovine or bovine fetus comprising a non-naturally occurring mutation in one or both alleles of an endogenous prion nucleic acid.
A method of producing a product that substantially lacks prion protein, said method comprising manufacturing said product in, or obtaining said product from, a bovine or bovine fetus comprising a non-naturally occurring mutation in one or both alleles of an endogenous prion nucleic acid.
A method of producing a cell comprising two genetic modifications, said method comprising the steps of:
- (a) providing a non-human mammalian somatic cell having a first genetic modification;
- (b) inserting said cell or a progeny thereof, a chromatin mass from said cell or progeny thereof, or a nucleus from said cell or progeny thereof into a nucleated or enucleated oocyte;
- (c) transferring said oocyte obtained from step (b) or an embryo formed from said oocyte into a recipient;
- (d) isolating a cell from said embryo, or from a fetus or juvenile produced therefrom, wherein said cell contains said first genetic modification;
- and
- (e) introducing a second genetic modification into the genome of said cell or a progeny thereof, thereby producing a cell comprising two genetic modifications.
A method of producing a cell comprising two genetic modifications, said method comprising the steps of:
- (a) providing a non-human mammalian somatic cell having a first genetic modification;
- (b) permeabilizing said somatic mammalian cell under conditions that allow chromatin condensation;
- (c) inserting said permeabilized cell into a nucleated or enucleated oocyte;
- (d) transferring said oocyte obtained from step (c) or an embryo formed from said oocyte into a recipient;
- (e) isolating a cell from said embryo, or from a fetus or juvenile produced therefrom, wherein said cell contains said genetic modification;
- and
- (f) introducing a second genetic modification into the genome of said cell or a progeny thereof, thereby producing a cell comprising two genetic modifications.
The mammal of claim 84, wherein said mammal is an ungulate.
Dependencies are inferred from the claim text.