US 2006/0040405 A1 · Test device and method for colored particle immunoassay

Abstract

Disclosed is a test cell and a method for detection of a preselected ligand in a liquid sample such as a body fluid. The test cell includes an elongate outer casing which houses an interior permeable material capable of transporting an aqueous solution and defining a sample inlet, a test volume, and a reservoir volume. The reservoir volume is disposed in a section of the test cell spaced apart from the inlet and is filled with sorbent material. The reservoir acts to receive liquid transported along a flow path defined by the permeable material and extending from the inlet and through the test volume. In the test volume is a test site which includes a first protein having a binding site specific to a first epitope of the ligand immobilized in fluid communication with the flow path. The test site can be observed through a window of the casing.

Claims26

Dependent claims

A method of detecting a ligand in a liquid sample, the method comprising the steps of:

A. transporting along a flow path in a test cell a solution, including a liquid sample suspected to contain a ligand and a conjugate, into contact with a test site visible through a window in a wall of said test cell,
said test site having immobilized thereon a first protein having a binding site specific to a first epitope on the ligand,
said conjugate comprising colored particles coupled to a second protein selected from the group consisting of proteins having a binding site specific to a second epitope on the ligand and proteins which bind with said first protein in competition with the ligand, and
B. continuing transport of said solution to progressively produce at said test site a complex comprising said ligand for a time sufficient to visually determine through said window whether a color is developed at said test site:

1 more element

Dependencies are inferred from the claim text.

Facts

Application

11/035,047Filed 12 Jan 2005

Priority

27 Jun 1988

Published

23 Feb 2006

Term ends

Not estimatedThe source records don’t support an estimate.

Classification20 codes

G01N 33/76
Involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and…:…
B01L 3/5023
With fluid transport, e.g. in multi-compartment structures: with a sample being transported to, and subsequently stored in an absorbent for analysis
G01N 33/54313
With an insoluble carrier for immobilising immunochemicals: the carrier being characterised by its particulate form
G01N 33/54366
With an insoluble carrier for immobilising immunochemicals: apparatus specially adapted for solid-phase testing

Family

Application07/211,582Continuation · filed 27 Jun 1988

Application07/702,450Continuation · filed 16 May 1991

Application07/995,331Continuation · filed 23 Dec 1992

Application08/886,088Continuation · filed 2 Jul 1997

Application09/441,875Continuation · filed 17 Nov 1999

Application11/035,047Continuation · filed 12 Jan 2005

US 5,714,389 ARelated patent

US 5,989,921 ARelated patent

US 2006/0040405 A1This patent

From the US filing history. Foreign family members aren’t listed.

Test device and method for colored particle immunoassay

Life of this patent

Test device and method for colored particle immunoassay

Life of this patent

Abstract

Claims26