Multi-lineage progenitor cells
AssigneeBioE, Inc.
InventorsDaniel Collins, Stacey Sprague, Barbara Tigges
Life of this patent
Estimating the term…
Abstract
Fetal blood multi-lineage progenitor cells that are capable of a wide spectrum of transdifferentiation are described.
Claims66
A purified population of human fetal blood multi-lineage progenitor cells (MLPC) or undifferentiated progeny thereof, wherein said MLPC have enhanced expression of mRNA for CXCR4, FLT3, and CD133 relative to that of a population of mesenchymal stem cells (MSC).
A clonal line of human fetal blood MLPC and undifferentiated progeny thereof, wherein said MLPC have enhanced expression of mRNA for CXCR4, FLT3, and CD 133 relative to that of a population of MSC.
A method for purifying a population of MLPC from human fetal blood, said method comprising:
- a) contacting a human fetal blood sample with a composition, said composition comprising:
- i) dextran;
- ii) anti-glycophorin A antibody;
- iii) anti-CD 15 antibody;
- and
- iv) anti-CD9 antibody;
- b) allowing said sample to partition into an agglutinate and a supernatant phase;
- c) recovering cells from said supernatant phase;
- d) purifying MLPC from the recovered cells by adherence to a solid substrate;
- and
- e) culturing said MLPC such that said MLPC obtain a fibroblast morphology, wherein said MLPC, after obtaining said fibroblast morphology, have enhanced expression of mRNA for CXCR4, FLT3, and CD133 relative to that of a population of MSC.
A method of characterizing a population of MLPC, said method comprising a) providing a purified population of MLPC;
- and b) assessing expression in said population of MLPC of one or more mRNAs selected from the group consisting of CXCR4, FLT3, CD133, ICAM2, ITGAX, TFRC, KIT, IL6R, IL7R, ITGAM, FLT3, PDGFRB, SELE, SELL, TFRC, ITGAL, ITGB2, PECAM1, ITGA2B, ITGA3, ITGA4, ITGA6, ICAM1, CD24, CD34, CD44, CD45, CD58, CD68, CD33, CD37, CD38, TERT, KIT, and POUF5.
A method for characterizing the immaturity of a population of MLPC, said method comprising a) providing a purified population of MLPC;
- and b) assessing expression of mRNA for CXCR4, FLT3, and CD133 in said population of MLPC, wherein enhanced expression of CXCR4, FLT3, and CD133 relative to that of a population of MSC is indicative of an immature phenotype.
A method of making a clonal line of MLPC, said method comprising
- a) providing a population of MLPC,
- b) culturing a single MLPC in a culture vessel lacking other cells until a plurality of progeny are produced;
- and
- c) culturing said progeny to obtain said clonal line.
Dependencies are inferred from the claim text.