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US 2006/0040370 A1Published application
Purified thermostable pyrococcus furiosus dna polymerase i
AssigneeStratagene
InventorEric Mathur
Life of this patent
Estimating the term…
Abstract
Purified thermostable Pyrococcus furiosus DNA polymerase that migrates on a non-denaturing polyacrylamide gel faster than phosphorylase B and Taq polymerase and more slowly than bovine serum albumin and has an estimated molecular weight of 90,000-93,000 daltons when compared with a Taq polymerase standard assigned a molecular weight of 94,000 daltons.
Claims7
1-11. (canceled)
An isolated polynucleotide encoding a thermostable DNA polymerase, wherein the isolated polynucleotide is capable of hybridizing to a polynucleotide complementary to a polynucleotide that encodes amino acids 1 to 775 of SEQ ID NO:
- 1 under hybridization conditions comprising incubating at 42° C. in a hybridization buffer comprising 0.9 M NaCl and 50% formamide, and under wash conditions comprising incubating at 68° C. in a wash buffer comprising 0.015 M NaCl and 0.5% sodium dodecyl sulfate.
17-30. (canceled)
Dependencies are inferred from the claim text.