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AssigneeGlycoFi, Inc.
InventorsRobert Davidson, Tillman Gerngross, Stefan Wildt, Byung-Kwon Choi, Juergen Nett, Piotr Bobrowicz, Stephen Hamilton
Estimating the term…
The present invention provides a novel lower eukaryotic host cell producing human-like glycoproteins characterized as having a terminal β-galactose residue and essentially lacking fucose and sialic acid residues. The present invention also provides a method for catalyzing the transfer of a galactose residue from UDP-galactose onto an acceptor substrate in a recombinant lower eukaryotic host cell, which can be used as a therapeutic glycoprotein.
A recombinant lower eukaryotic host cell producing human-like glycoproteins characterized as having a terminal β-galactose residue and essentially lacking fucose and sialic acid residues on the glycoprotein.
A recombinant lower eukaryotic host cell producing human-like glycoproteins, the host comprising an isolated nucleic acid molecule encoding β-galactosyltransferase activity and at least an isolated nucleic acid molecule encoding UDP-galactose transport activity, UDP-galactose C4 epimerase activity, galactokinase activity or a galactose-1-phosphate uridyl transferase activity.
A recombinant lower eukaryotic host cell producing human-like glycoproteins, the host cell capable of transferring β-galactose residue onto an N-linked oligosaccharide branch of a glycoprotein comprising a terminal GlcNAc residue, the N-linked oligosaccharide branch selected from the group consisting of GlcNAcβ1,2-Manα1,3;
A composition comprising a human-like glycoprotein characterized as having a terminal β-galactose residue and essentially lacking fucose and sialic acid residues on the glycoprotein.
A method for producing human-like glycoproteins in a lower eukaryotic host cell the method comprising the step of producing UDP-galactose above endogenous levels.
A method for producing human-like glycoprotein composition in lower eukaryotic host cell comprising the step of transferring a galactose residue on a hybrid or complex glycoprotein in the absence of fucose and sialic acid residues.
A recombinant lower eukaryotic host cell expressing GalNAc Transferase activity.
A recombinant lower eukaryotic host cell expressing a gene encoding heterologous UDPase activity.
An isolated polynucleotide comprising or consisting of a nucleic acid sequence selected from the group consisting of:
A modified polynucleotide comprising or consisting of a nucleic acid sequence selected from the group consisting of the conserved regions of SEQ ID NO:
Dependencies are inferred from the claim text.