Thermophilic hydrophobin proteins and applications for surface modification
AssigneeE. I. Du Pont de Nemours and Company
InventorsJames Sweigard, Barry Stieglitz
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Abstract
The present invention relates to a thermophilic hydrophobin, TT1, or a protein or polypeptide substantially similar thereto, derived from the thermophilic fungus Talaromyces thermophilus. The invention further relates to a polynucleotide encoding such hydrophobin, as well as to materials coated with such hydrophobin.
Claims37
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a hydrophobin polypeptide, wherein the polypeptide has an amino acid sequence of at least 80% sequence identity, based on the Clustal W method of alignment, when compared to SEQ ID NO:1, or
- (b) a complement of the nucleotide sequence of (a), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100%-complementary.
An isolated hydrophobin polypeptide, wherein the polypeptide has an amino acid sequence of at least 80% sequence identity, based on the Clustal W method of alignment, when compared to SEQ ID NO:1.
A composite structure comprising a polymer material having a thermophilic hydrophobin coating thereon, said hydrophobin having the amino acid sequence as set forth in SEQ ID NO:1.
A composite structure comprising a polymer material having a thermophilic hydrophobin coating thereon wherein the wettability is altered as characterized by a difference in water contact angle of at least about 20°.
A method of treating the surface of an object with a thermophilic hydrophobin containing solution comprising:
- a) roviding a surface;
- b) ontacting the surface of (a) with a thermophilic hydrophobin at a temperature of at least about 25° C. to at least about 100° C., wherein the surface is coated.
Dependencies are inferred from the claim text.