Methods for screening polypeptides
AssigneeAffymetrix, Inc.
InventorsFred Christians, Kyle Cole
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Abstract
In one aspect of the invention, methods are provided for the creation and screening of polypeptides that eliminates bacterial cloning and individual screening In preferred embodiments, the method involves partnering each protein with a unique DNA oligonucleotide tag that directs the protein to a unique site on the microarray due to specific hybridization with a complementary tag-probe on the array
Claims21
A method for screening a plurality of polypeptides comprising
- linking each of the plurality of polypeptides with a nucleic acid tag to obtain tagged polypeptides,
- hybridizing the tagged polypeptides with an oligonucleotide probe array to immobilize the tagged polypeptides on the array, wherein the oligonucleotide probe array has at least one probe for each of the nucleic acid tag, and screening the polypeptides for an activity
The method of claims 2, 3, 4, 5 or 6 wherein the oligonucleotide probe array has at least 400 different oligonucleotide probes per cm2
A method for screening a plurality of polypeptides comprising
- attaching oligonucleotide tags to a plurality of mRNAs,
- hybridizing the plurality of mRNAs to an oligonucleotide array, wherein the oligonucleotide array has at least one probe for each of the oligonucleotide tags,
- translating the mRNAs to produce the plurality of polypeptides, wherein the translation is performed under the condition that the resulting peptides are attached to the mRNA, and
- screening the polypeptides for an activity
Dependencies are inferred from the claim text.