US 2006/0040305 A1 · Non-invasive prenatal genetic diagnosis using transcervical cells

Abstract

A non-invasive, risk-free method of prenatal diagnosis is provided. According to the method of the present invention cell specimens are subjected to molecular and morphological methods which allow trophoblast identification. Trophoblasts identified according to the teachings of the present invention can be further examined to thereby prenatally diagnosing a fetus. Also provided is a method of in situ chromosomal, DNA and/or RNA analysis of a prestained specimen by incubating the prestained specimen in ammonium hydroxide. Also provided is a method of identifying embryonic cells according to a nucleus/cytoplasm ratio of at least 0.3 and the presence of at least variably condensed chromatin.

Claims48

Dependent claims

A method of diagnosing and/or determining a gender of a fetus, the method comprising:

(a) combining molecular and morphological methods to identify at least one trophoblast;
and
(b) examining said at least one trophoblast, thereby diagnosing and/or determining the gender of the fetus.

Dependencies are inferred from the claim text.

Facts

Application

11/206,139Filed 18 Aug 2005

Priority

3 Apr 2003

Published

23 Feb 2006

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

G01N 33/689
Involving proteins, peptides or amino acids: related to pregnancy or the gonads
C12Q 1/6841
Hybridisation assays: in situ hybridisation
C12Q 1/6879
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for sex determination
C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material

Family

Application10/405,698Continuation · filed 3 Apr 2003

Application10/921,899Continuation in part · filed 20 Aug 2004

Application11/088,882Continuation in part · filed 25 Mar 2005

Application11/206,139Continuation in part · filed 18 Aug 2005

US 2004/0197832 A1Published application

US 2005/0003351 A1Published application

US 2005/0181429 A1Published application

US 2006/0040305 A1This patent

From the US filing history. Foreign family members aren’t listed.

Non-invasive prenatal genetic diagnosis using transcervical cells

Life of this patent

Non-invasive prenatal genetic diagnosis using transcervical cells

Life of this patent

Abstract

Claims48