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US 2006/0040270 A1Published application
Processes for detecting or quantifying more than one nucleic acid in library
AssigneeEnzo Life Sciences, Inc.
InventorsElazar Rabbani, Jannis Stavrianopoulos, James Donegan, Jack Coleman
Life of this patent
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Abstract
This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
Claims87
(canceled)
2-251. (canceled)
A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of:
- a) providing:
- (i) an array of fixed or immobilized nucleic acids identical in part or whole to said nucleic acids of interest;
- (ii) a library of nucleic acid analytes which may contain the nucleic acids of interest sought to be detected or quantified, wherein each of said nucleic acids of interest comprise at least one inherent universal detection target (UDT);
- (iii) universal detection elements (UDE) which generate a signal directly or indirectly;
- and
- (iv) polymerizing means for synthesizing nucleic acid copies of said nucleic acids of analytes;
- b) synthesizing one or more first nucleic acid copies which are complementary to all or part of said nucleic acid analytes and synthesizing sequences which are complementary to all or part of said UDT to form a complementary UDT;
- c) hybridizing said first nucleic acid copies with said array of nucleic acids (i) to form hybrids if said nucleic acids of interest are present;
- d) contacting said UDEs with said complementary UDTs of said first nucleic acid copies to form a complex bound to said array;
- e) detecting or quantifying said more than one nucleic acid of interest by detecting or measuring the amount of signal generated from UDEs bound to said array.
7 more elements
The process of claim. 252, wherein said solid support is porous or non-porous.
A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of:
- a) providing:
- (i) an array of fixed or immobilized nucleic acids identical in part or whole to said nucleic acids of interest;
- (ii) a library of nucleic acid analytes which may contain the nucleic acids of interest sought to be detected or quantified, wherein each of said nucleic acids of interest comprise at least one inherent universal detection target (UDT);
- (iii) universal detection elements (UDE) which generate a signal directly or indirectly;
- and
- (iv) polymerizing means for synthesizing nucleic acid copies of said nucleic acid analytes;
- b) synthesizing one or more first nucleic acid copies of said nucleic acid analytes;
- c) contacting said UDEs with said UDTs in said first nucleic acid copies to form one or more complexes;
- d) hybridizing said first nucleic acid copies with said array of nucleic acids (i) to form hybrids if said nucleic acids of interest are present;
- and
- e) detecting or quantifying said more than one nucleic acid of interest by detecting or measuring the amount of signal generated from UDEs bound to said array.
8 more elements
A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of:
- a) providing:
- (i) an array of fixed or immobilized nucleic acids identical in part or whole to said nucleic acids of interest;
- (ii) a library of nucleic acid analytes which may contain the nucleic acids of interest sought to be detected or quantified;
- (iii) means for attaching one or more non-inherent universal detection targets (UDT) to a nucleic acid;
- (iv) universal detection elements (UDE) which generate a signal directly or indirectly;
- and
- (v) polymerizing means for synthesizing nucleic acid copies of said nucleic acid analytes;
- b) attaching said non-inherent UDTs to either the 3′ ends of said nucleic acid analytes, the 5′ ends of said first nucleic acid analytes, or both said 3′ ends and said 5′ ends of said nucleic acid analytes;
- c) synthesizing one or more first nucleic acid copies of said nucleic acid analytes;
- d) hybridizing said first nucleic acid copies with said array of nucleic acids (i) to form hybrids if said nucleic acids of interest are present;
- e) contacting said UDEs with said UDTs of said first nucleic acid copies to form a complex bound to said array;
- f) detecting or quantifying said more than one nucleic acid of interest by detecting or measuring the amount of signal generated from UDEs bound to said array.
9 more elements
A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of:
- a) providing:
- (i) an array of fixed or immobilized nucleic acids identical in part or whole to said nucleic acids of interest;
- (ii) a library of nucleic acid analytes which may contain the nucleic acids of interest sought to be detected or quantified;
- (iii) means for attaching one or more non-inherent universal detection targets (UDT) to a nucleic acid;
- (iv) universal detection elements (UDE) which generate a signal directly or indirectly;
- and
- (v) polymerizing means for synthesizing nucleic acid copies of said nucleic acid analytes;
- b) attaching said non-inherent UDTs to either the 3′ ends of said nucleic acid analytes, the 5′ ends of said first nucleic acid analytes, or both said 3′ ends and said 5′ ends of said nucleic acid analytes;
- c) synthesizing one or more first nucleic acid copies of said nucleic acid analytes;
- d) contacting said UDEs with said UDTs of said first nucleic acid copies to form complexes;
- e) hybridizing said first nucleic acid copies with said array of nucleic acids (i) to form hybrids if said nucleic acids of interest are present;
- f) detecting or quantifying said more than one nucleic acid of interest by detecting or measuring the amount of signal generated from UDEs bound to said array.
9 more elements
336-952. (canceled)
Dependencies are inferred from the claim text.