Nucleotide sequences specific to Francisella tularensis and methods for the detection of Francisella tularensis

A method comprising:

• (i) providing a sample;
• (ii) forming a mixture by adding the sample to a solution containing at least one series of nucleotide sequences having a forward primer, a reverse primer and a hybridization probe selected from the group consisting of SEQ ID NOs:1, 2, 3;
• 5, 6, 7;
• 9, 10, 11;
• 13, 14, 15;17, 18, 19;
• 21, 22, 23;
• 25, 26, 27;29, 30, 31;
• under conditions suitable for isolating genomic DNA for amplification using PCR and under conditions suitable for hybridization with said at least one series of nucleotide sequences;
• and
• (iii)subjecting the mixture to PCR:

A method comprising

• (i) providing a sample;
• (ii) forming a mixture by adding the sample to a solution containing at least one series of nucleodtide sequences having a forward primer, a reverse primer and a hybridization probe selected from the group consisting of SEQ ID NOs:1, 2, 3;
• 5, 6, 7;
• 9, 10, 11;
• 13, 14, 15;17, 18, 19;
• 21, 22, 23;
• 25, 26, 27;29, 30, 31;
• under conditions suitable for isolating genomic DNA for amplification using PCR and under conditions suitable for hybridization with said at least one series of nucleotide sequences;
• and
• (iii) detecting the presence of at least one Amplicon sequence by flurogenic 5′ nuclease PCR assay, wherein the presence of said one Amplicon sequence indicates the existence of Francisella tularensis in the sample.