Immunoassay for HIV protease inhibitors
OwnerSalvatore Salamone, Gerald Sigler +1inventors
InventorsSalvatore Salamone, Gerald Sigler, Lili Arabshahi
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Abstract
A non-isotopic immunoassay for an HIV protease inhibitor comprising incubating a sample containing the inhibitor with a receptor specific for the inhibitor or for a metabolite of said inhibitor and further with a conjugate comprising an analog of the inhibitor and a non-isotopic signal generating moiety. Signal generated as a result of binding of the inhibitor by the receptor is measured and correlated with the presence or amount of protease inhibitor in the original sample.
Claims11
An immunoassay for determining an HIV protease inhibitor in a sample comprising the steps of:
- (a) combining a sample suspected of containing said protease inhibitor with a receptor specific for said inhibitor and a conjugate comprising a ligand of said inhibitor and a non-isotopic signal generating moiety,
- (b) measuring the amount of said receptor bound to said conjugate by monitoring the production of signal generated by said moiety, and
- (c) correlating said production of signal with the presence or amount of said inhibitor in said sample.
A non-isotopic immunoassay for determining an HIV protease inhibitor in a sample comprising the steps of:
- (a) combining a sample suspected of containing said protease inhibitor with a conjugate comprising a ligand of said protease inhibitor and mycophenolic acid, a receptor specific for said protease inhibitor, IMP, NAD and IMPDH,
- (b) monitoring the production of NADH, and
- (c) correlating the production of NADH with the presence or amount of said protease inhibitor in said sample.
A test kit for determining an HIV protease inhibitor in a sample comprising in packaged combination:
- (a) a receptor specific for said inhibitor and
- (b) a conjugate comprising a ligand of said inhibitor and a non-isotopic signal generating moiety.
Dependencies are inferred from the claim text.