Ex-vivo rescue of hematopoietic stem cells after lethal irradiation
AssigneeLarge Scale Biology Corporation
InventorJohn Chute
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Abstract
A method of autologous rescue of an individual exposed to a harmful hematopoietic stem cell condition is described. The method includes removing hematopoietic stem cells from the individual, culturing the hematopoietic stem cells and reintroducing cultured hematopoietic stem cells into the individual. Stem cells are grown in a stromal-cell free medium. The harmful condition can be radiation. The culture medium can contain any of several growth stimulating factors.
Claims20
A method for autologous rescue of an individual exposed to a harmful hematopoietic stem cells condition comprising;
- removing hematopoietic stem cells from said individual,
- culturing the hematopoietic stem cells in a stromal-cell free medium, and
- reintroducing cultured hematopoietic stem cells into said individual.
A composition comprising viable hematopoietic stem cells previously exposed to a harmful hematopoietic stem cell condition, a cell culture medium, stem cell factor and flt-3 ligand.
A method for culturing hematopoietic stem cells previously exposed to a harmful hematopoietic stem cell condition comprising;
- contacting said cells in a cell culture medium containing cytokines, and
- incubating said cells in said medium,
- wherein the hematopoietic stem cells remain viable.
Dependencies are inferred from the claim text.