System and method for determining radius of gyration, molecular weight, and intrinsic viscosity of a polymeric distribution using gel permeation chromatography and light scattering detection

On a computer system, a method of determining the conformation law of a sample processed by gel permeation chromatography, said method comprising the steps of:

• obtaining data from a detector detecting data from at least two channels of a sample;
• obtaining a parameterized model describing sample properties;
• choosing initial valves for parameters from said parameterized model;
• constructing an initial curve from said data, from said parameterized model, and from said initial values;
• determining a best fit curve and best fit parameter values from said initial curve and from said parameterized model to determine said radius of gyration distribution;
• obtaining second data from a second detector detecting said sample;
• and
• determining a best fit curve and best fit parameter values from said initial curve, from said parameterized model and from said second data to provide a MWD of said sample thereby determining the conformation law of said sample.

On a computer system, a method of determining the IVL of a sample processed by gel permeation chromatography, said method comprising the steps of:

• obtaining first data from a first detector detecting said sample;
• obtaining a parameterized model describing log intrinsic viscosity versus log molecular weight;
• choosing initial values for parameters for said parameterized model;
• obtaining hydrodynamic volume of slices from a universal calibration curve;
• constructing an initial curve of specific viscosity from said first data, from said parameterized model, from said initial values, and from said hydrodynamic volume of slices;
• obtaining second data from a second detector detecting at least two channels from said sample;
• and
• determining a best fit curve of specific viscosity and best fit parameter values of said model of log intrinsic viscosity versus log molecular weight from said initial curve, from said second parameterized model, and from said second data;
• and
• determining IVL from said parameterized model and said best fit parameter.

On a computer system, a method of determining radius of gyration, conformational law, molecular weight distribution, intrinsic viscosity and the column calibration of a sample processed by gel permeation chromatography, said method comprising the steps of:

• obtaining first data from a first detector detecting said sample;
• obtaining a parameterized model describing log intrinsic viscosity versus log molecular weight;
• choosing initial values for parameters for said parameterized model;
• obtaining hydrodynamic volume of slices from a universal calibration curve;
• constructing an initial curve of specific viscosity from said first data, from said parameterized model, from said initial values, and from said hydrodynamic volume of slices;
• obtaining second data from a second detector detecting at least two channels from said sample;
• obtaining third data from a third detector detecting concentration of said sample;
• and
• determining a best fit curve of specific viscosity and best fit parameter values of said model of log intrinsic viscosity versus log molecular weight from said initial curve, from said second parameterized model, from said second data and from said third data;
• and
• determining the radius of gyration, IVL, conformational law, intrinsic viscosity and column calibration from said parameterized model and said best fit parameter.