Method for purifying factor VIII/vWF-complex by means of cation exchange chromatography
AssigneeBaxter Aktiengesellschaft
InventorsArtur Mitterer, Bernhard Fischer, Oyvind Schonberger, Kathrin Thomas-Urban, Friedrich Dorner, Johann Eibl
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Abstract
There is disclosed a method of recovering factor VIII/vWF-complex which is characterized in that factor VIII/vWF-complex from a protein solution is bound to a cation exchanger and is recovered by step-wise elution of factor VIII/vWF-complex , which particularly contains high-molecular vWF multimers, as well as a factor VIII/vWF-complex obtainable by means of cation exchange chromatography.
Claims8
A method of recovering factor VIII/vWF-complex, characterized in that factor VIII/vWF-complex from a protein solution is bound to a cation exchanger and is recovered by step-wise elution of factor VIII/vWF-complex, which particularly contains high-molecular vWF-multimers.
2-16. (canceled)
Factor VIII:C, substantially free from platelet agglutinating vWF activity, obtainable from a factor VIII/vWF-containing solution by cation exchange chromatography and step-wise elution at a salt concentration of between ≧200mM and ≦300mM.
A method of treating patents suffering from at least one of hemophilia A, phenotypic hemophilia and vWD, comprising administering to said patients an effective dose of a factor VIII/vWF-complex having specific vWF activity of at least 66 U/mg protein and a specific factor VIII activity of at least 500 U/mg protein, wherein said factor VIII/vWF-complex is virus safe and free from infectious material.
A method of treating patients suffering from at least one of hemophilia A, phenotypic hemophilia and vWF, comprising administering to said patients an effective dose of a factor VIII:C obtainable from a factor VIII/vWF-containing solution by cation exchange chromatography and step-wise elution at a salt concentration of between ≧200mM and ≦300mM, wherein said factor VIII:C is virus safe and free from infectious material.
Dependencies are inferred from the claim text.