Loading patent…
AssigneeOregon Health & Science University
InventorsSteven Hefeneider, Robert Bennett
Estimating the term…
The present invention relates to novel mammalian DNA-R proteins and genes that encode such proteins. The invention is directed toward the isolation and characterization of mammalian DNA-R proteins. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to rat and human homologues of a mammalian DNA-R gene. Also provided are recombinant expression constructs capable of expressing the mammalian DNA-R genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the mammalian catecholamine receptor proteins encoded therein. The invention also provides methods for screening compounds in vitro that are capable of binding to the mammalian DNA-R proteins of the invention, and further characterizing the binding properties of such compounds in comparison with known DNA-R agonists and antagonists. Improved methods of pharmacological screening are provided thereby.
A homogeneous composition of a mammalian DNA-R or derivative thereof having a molecular weight of about 150 kilodaltons and an amino acid sequence identified by SEQ ID No.:2.
A homogeneous composition of a DNA-binding fragment of a mammalian DNA-R or derivative thereof having a molecular weight of about 63 kilodaltons and an amino acid sequence identified by amino acids 1-575 of SEQ ID No.:2.
A homogeneous composition of a soluble mammalian DNA-R or derivative thereof having a molecular weight of about 145 kilodaltons and an amino acid sequence identified by SEQ ID No.:2 wherein amino acids 1133-1171 are deleted therefrom.
A cell membrane preparation comprising a mammalian DNA-R or derivative thereof having a molecular weight of about 150 kilodaltons and an amino acid sequence identified by SEQ ID No.:2.
A cell membrane preparation comprising a mammalian DNA-R or derivative thereof having a molecular weight of about 63 kilodaltons and an amino acid sequence identified by amino acids 1-575 of SEQ ID No.:2.
A cell membrane preparation comprising a mammalian DNA-R or derivative thereof having a molecular weight of about 145 kilodaltons and an amino acid sequence identified by SEQ ID No.:2 wherein amino acids 1133-1171 are deleted therefrom.
Dependencies are inferred from the claim text.