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US 2005/0233457 A1Published application
Recombining viral vectors for the tetracycline-regulated expression of genes
OwnerAndreas Blockinventor
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Abstract
The invention relates to recombining viral vectors which can be suppressed in a highly efficient manner by means of tetracycline or tetracycline derivatives, and the use thereof for expressing genes in eukaryotic cells, particularly within the framework of gene therapy.
Claims18
Recombinant viral vector which contains an insert exhibiting the general structure
- tTA-intron1-TK+-TetO7-CMV+-intron2-transgene
- in which
- TetO7 is the heptamerized tetracycline operator
- TK+ is the minimal thymidine kinase promoter
- tTA is a nucleic acid sequence which encodes a fusion protein from the repressor protein inducible by tetracycline and the transcriptional activation domain of the Herpes simplex virus VP16,
- CMV+ is the minimal cytomegalovirus promoter and
- Transgene is a nucleic acid sequence which codes for a non-viral protein
- Intron1 is any desired non-encoding nucleic acid sequence with a length of O to approximately 1000 bp and
- Intron2 is any desired non-encoding nucleic acid sequence with a length of O to approximately 1000 bp.
6 more elements
Expression plasmid with the nucleic acid sequence represented in SEQ ID NO:4 or SEQ ID NO:5.
Dependencies are inferred from the claim text.