Simple and rapid derivation of functional hepatocytes from human bone marrow-derived mesenchymal stem cells
OwnerKuan-Der Lee, Jacqueline Whang-Penginventors
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Abstract
This disclosure provides methods for preparing hepatocytes from mesenchymal stem cells by culturing in a first culture media comprising hepatocyte growth factor and a second culture media comprising oncostatin-M. The disclosure also provides the MSC-derived hepatocytes produced by these methods and both in vivo and in vitro uses for these MSC-derived hepatocytes.
Claims29
A method for inducing differentiation of mesenchymal stem cells into hepatocytes in vitro comprising incubating cultured mesenchymal stem cells with a first culture media comprising hepatocyte growth factor followed by incubating the cells with a second culture media comprising oncostatin-M.
A composition comprising mesenchymal stem cell (MSC)-derived hepatocytes.
A method for repairing liver damage in a patient, comprising administering MSC-derived hepatocytes.
A method for growing liver tissue in vitro comprising repeated culturing and expansion of MSC-derived hepatocytes.
A kit for the preparation of MSC-derived hepatocytes comprising a first culture media comprising hepatocyte growth factor, and a second culture media comprising oncostatin-M.
A kit for the in vitro growth of liver-specific viruses comprising MSC-derived hepatocytes.
Dependencies are inferred from the claim text.