Recombinant narbonolide polyketide synthase
OwnerGary Ashley, Melanie Betlach +3inventors
InventorsGary Ashley, Melanie Betlach, Mary Betlach, Robert McDaniel, Li Tang
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Abstract
Recombinant DNA compounds that encode all or a portion of the narbonolide polyketide synthase are used to express recombinant polyketide synthase genes in host cells for the production of narbonolide, narbonolide derivatives, and polyketides that are useful as antibiotics and as intermediates in the synthesis of compounds with pharmaceutical value.
Claims20
An isolated recombinant DNA compound that comprises a coding sequence for a domain of a narbonolide PKS.
An isolated recombinant DNA compound that comprises a coding sequence for a desosamine biosynthetic gene or a desosaminyl transferase gene or a beta-glucosidase gene of Streptomyces venezuelae.
An isolated recombinant DNA compound that comprises a coding sequence for a picK hydroxylase gene of Streptomyces venezuelae.
A method for increasing the yield of a desosaminylated polyketide in a cell, which method comprises transforming the cell with a recombinant expression vector that encodes a functional beta-glucosidase gene.
Dependencies are inferred from the claim text.