Nucleic acid molecules from rice controlling abiotic stress tolerance
AssigneeSyngenta Participations AG
InventorsHenry Nguyen, Joel Kreps
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Abstract
The present subject matter provides a method for enhancing the abiotic stress tolerance of a plant. Polynucleotides isolated from rice (Oryza sativa) and encoding polypeptides for abiotic stress tolerance are also described.
Claims32
An isolated polypeptide selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence of one of even numbered SEQ ID NOs:
- 2-196;
- (b) a polypeptide having an amino acid sequence that is at least 60% identical to and having a same function as a polypeptide having an amino acid sequence of one of even numbered SEQ ID NOs:
- 2-196;
- (c) a polypeptide having an amino acid sequence encoded by a nucleotide sequence that is substantially identical to a nucleotide sequence of one of odd numbered SEQ ID NOs:
- 1-195;
- (d) a polypeptide having an amino acid sequence encoded by a nucleic acid molecule capable of hybridizing under highly stringent conditions to a nucleic acid molecule of one of odd numbered SEQ ID NOs:
- 1-195 or to a sequence fully complementary thereto;
- and
- (e) a functional fragment of a polypeptide encoded by a nucleic acid molecule encoding the amino acid sequence of (a), (b), (c) or (d).
An isolated nucleic acid molecule comprising a nucleotide sequence, its full complement, or its full reverse complement, the nucleotide sequence encoding a polypeptide selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence listed in even numbered sequences of SEQ ID NOs:
- 2-196, or a functional fragment, domain, repeat, or feature thereof;
- (b) a polypeptide having an amino acid sequence that is at least 60% identical to and having a same function as a polypeptide having an amino acid sequence of one of even numbered SEQ ID NOs:
- 2-196;
- (c) a polypeptide having an amino acid sequence encoded by a nucleotide sequence having at least 60% identity to a nucleotide sequence listed in odd numbered sequences of SEQ ID NOs:
- 1-195, or a functional fragment, domain, or feature thereof, or a sequence fully complementary thereto;
- and
- (d) a polypeptide having an amino acid sequence encoded by a nucleotide sequence capable of hybridizing under medium stringency conditions to a nucleotide sequence listed in odd numbered sequences of SEQ ID NOs:
- 1-195, or to a sequence fully complementary thereto;
An isolated nucleic acid molecule comprising a nucleotide sequence, the nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence listed in odd numbered sequences of SEQ ID NOs:
- 1-195, or a functional fragment, domain, or feature thereof;
- (b) a nucleotide sequence that is at least 60% identical to (a);
- (c) a nucleotide sequence capable of hybridizing to (a) under medium stringency conditions;
- (d) a nucleotide sequence complementary to (a), (b) or (c);
- and
- (e) a nucleotide sequence which is the reverse complement of (a), (b) or (c).
A shuffled nucleic acid comprising a plurality of nucleotide sequence fragments, wherein at least one of the fragments corresponds to a region of a nucleotide sequence listed in odd numbered sequences of SEQ ID NOs:
- 1-195, and wherein at least two of the plurality of sequence fragments are in an order, from 5′ to 3′, which is not an order in which the plurality of fragments naturally occur in a nucleic acid.
An isolated product from a plant comprising an expression cassette comprising a promoter sequence operatively linked to a nucleic acid, the nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence listed in odd numbered sequences of SEQ ID NOs:
- 1-195, or a functional fragment, domain, or feature thereof;
- (b) a nucleotide sequence encoding a polypeptide listed in even numbered sequences of SEQ ID NOs:
- 2-196;
- (c) a nucleotide sequence that is at least 60% identical to (a) or (b);
- (d) a nucleotide sequence capable of hybridizing to (a) or (b) under medium stringency conditions;
- (e) a nucleotide sequence fully complementary to (a), (b), (c) or (d);
- and
- (f) a nucleotide sequence that is the full reverse complement of (a), (b), (c) or (d).
A method for producing a recombinant polypeptide, the method comprising:
- (a) growing recombinant cells comprising a nucleic acid construct under suitable growth conditions, the construct comprising an expression vector and a nucleic acid comprising a nucleic acid selected from the group consisting of:
- (i) a nucleic acid encoding a polypeptide as listed in even numbered nucleotide sequences of SEQ ID NOs:
- 2-196;
- (ii) a nucleic acid sequence listed in odd numbered nucleotide sequences of SEQ ID NOs:
- 1-195;
- and
- (iii) a subsequence of (i) or (ii);
- and
- (b) isolating from the recombinant cells the recombinant polypeptide expressed therein.
Dependencies are inferred from the claim text.