Use of antibody from lymphocyte secretions to diagnose active infection
AssigneeICDDR, B: The Center for Health and Population Research
InventorsDavid Sack, Rubhana Raquib
Life of this patent
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Abstract
A method and kit for the detection of an infectious disease, particularly tuberculosis, wherein lymphocytes of a subject are incubated with a disease-specific antigen, and the level of antibody production is measured, the production of antibodies above a baseline level being indicative of infection.
Claims25
A method of diagnosing tuberculosis in a subject comprising culturing lymphocytes from said subject under suitable conditions such that antibodies are produced, and measuring the concentration of antibodies reactive with a tuberculosis antigen, wherein an increase in said concentration over an indicated cutoff value is indicative of active tuberculosis infection.
A method of diagnosing tuberculosis in a subject comprising
- v) obtaining a blood sample from said subject;
- vi) separating lymphocytes from the sample;
- vii) culturing the lymphocytes in a culture medium under suitable conditions;
- viii) measuring the concentration of antibodies reactive with a tuberculosis antigen in said medium;
- wherein an increase in said concentration over an indicated cutoff value is indicative of active tuberculosis infection.
A method of diagnosing active infection in a subject comprising culturing lymphocytes from said subject under suitable conditions such that antibodies are produced and measuring the concentration of antibodies reactive with a target disease antigen, wherein an increase in said concentration over a normal control sample is indicative of active infection with the target disease.
A method of diagnosing active infection of a target disease in a subject, said method comprising measuring the number of lymphocytes present in a blood sample obtained from said subject that secret antibodies reactive with a specific target disease antigen, wherein an increase in said number over a normal control sample is indicative of active infection with the target disease.
A kit for detection of active infection of a target disease in a subject, said kit comprising an antigen specific for said target disease suitable for eliciting an antibody response from cultured lymphocytes, and optionally suitable reagents for lymphocyte culture.
Dependencies are inferred from the claim text.