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AssigneeThe Regents of the University of California
InventorsPalmer Taylor, Zoran Radie, Jian Shi, Aileen Boyd
Estimating the term…
Disclosed are methods for the preparation and use of labeled AChE and labeled AChE inhibitory conjugate compositions for detecting accumulation of toxic materials such as organophosphates, insecticides, and other nerve agents. Also disclosed are methods for the use of labeled AChE and labeled AChE inhibitory conjugate compositions in a variety of areas, including the detecting of toxic materials in biological samples, in the area of food and water analysis, in environmental monitoring, and in industrial settings.
An in vitro method for identifying an acetylcholinesterase (AChE) cognate partner comprising;
A device for measuring accumulation of a conjugate between acetylcholinesterase (AChE) and a cognate partner comprising labeled AChE, wherein at least one site of the AChE is labeled peripheral to the active site of the enzyme.
A labeled acetylcholinesterase (AChE) molecule comprising at least one fluorophore present at the periphery of the active site of AChE, wherein the at least one site is selected from the group consisting of residues 76, 81, 84, 124, 262 and 287 of AChE or equivalents thereof.
A conjugate comprising a labeled acetylcholinesterase (AChE) molecule, an inhibitor of AChE and an antibody.
A method for evaluating the presence of an inhibitor of acetylcholinesterase (AChE), wherein the inhibitor is selected from the group consisting of carbamates and organophosphates, comprising the steps of:
A method for evaluating the presence of an inhibitor of acetylcholinesterase (AChE), wherein the inhibitor is selected from the group consisting of carbamates and organophosphates, comprising the steps of:
A kit comprising:
Dependencies are inferred from the claim text.