US 2005/0033038 A1 · Process for preparing haptens for immunoassay of phosphorothioate pesticides

Abstract

The present invention relates to a process for preparing haptens for immunoassay of phosphorothioate pesticides, which comprises the steps of reacting O-methyl(ethyl) dichlorothiophosphate with a phenolic compound to obtain O-methyl(ethyl) O-aryl chlorothiophosphate, and reacting the O-(methyl)ethyl O-aryl chlorothiophosphate thus obtained with aminocarboxylic acid to give desired haptens. In accordance with the present invention, haptens having a structure of O-methyl(ethyl) O-aryl, N-(carboxyalkyl)phosphoramidothioate or O-methyl(ethyl) O-aryl N-alkyl-N-(carboxyalkyl)phosphoramidothioate can be simply prepared with a high yield by employing two-step processes in a cost-efficient manner.

Claims3

Dependent claims

A process for preparing haptens for immunoassay of organophosporous phosphorothioate pesticides, which comprises the steps of:

(i) reacting a compound(10) with a phenolic compound(11) and K2CO3 in acetonitrile at 4° C. for 30 to 90 min to obtain a compound(12);
and,
(ii) reacting the compound(12) with a compound(13) and KOH in methanol at 4° C. for 3 to 5 min to obtain a compound(14)
wherein,
R1 is methyl or ethyl group;
R2 is aryl group;
R3 is hydrogen or alkyl group;
and,
R4 is polymethylene or substituted polymethylene group.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/876,748Filed 25 Jun 2004

Priority

KR 10-2001-008738228 Dec 2001

Published

10 Feb 2005

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

G01N 33/5308
Immunoassay; Biospecific binding assay; Materials therefor: for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
C07F 9/2458
The amide moiety containing a substituent or a structure which is considered as characteristic: of aliphatic amines
C07F 9/58
Six-membered rings: pyridine rings
G01N
Investigating or analysing materials by determining their chemical or physical properties

Process for preparing haptens for immunoassay of phosphorothioate pesticides

Life of this patent

Process for preparing haptens for immunoassay of phosphorothioate pesticides

Life of this patent

Abstract

Claims3