Testis-specific differentiation-regulatory factor
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsTakashi Sugihara, Renu Wadhwa, Sunil Kaul, Youji Mitsui
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Abstract
A gene expressed specifically in the testis has been unexpectedly isolated in the course of studies of the expression of a gene encoding an unknown protein that triggers cell death. The isolated gene was a novel gene sequence that had no significant homologue in the database. This gene was also found to be involved in the regulation of differentiation in the testis.
Claims9
An isolated DNA encoding a protein comprising SEQ ID NO:
- 4 or 5.
An isolated DNA specifically hybridizing to a DNA comprising the nucleotide sequence of any one of SEQ ID NOs:
- 1 to 3, and comprising at least 15 nucleotides.
An isolated DNA encoding a protein comprising an amino acid sequence in which one of several amino acids of SEQ ID NO:
- 4 or 5 have been replaced, deleted, and/or added, and which is functionally equivalent to a protein comprising SEQ ID NOs:
- 4 or 5.
An isolated DNA encoding a protein which is encoded by a DNA hybridizing under stringent conditions to the DNA comprising the nucleotide sequence of SEQ ID NO:
- 1 or 3, wherein the protein has testicular differentiation activity.
A method of producing a protein comprising:
- culturing a transformant comprising DNA which hybridizes under stringent conditions to a DNA comprising a nucleotide of SEQ ID NO:
- 1 or 3 under conditions where a protein is expressed;
- and
- obtaining the protein from the transformant or the culture supernatant thereof.
Dependencies are inferred from the claim text.