Immunoglobulin chimeric monomer-dimer hybrids

A chimeric protein comprising a first and second polypeptide chain, wherein said first chain comprises a biologically active molecule, and at least a portion of an immunoglobulin constant region and

• wherein said second chain consists of at least a portion of an immunoglobulin constant region and optionally an affinity tag.

The chimeric protein of 28, wherein the affinity tag is a FLAG tag.

A chimeric protein comprising a first and second polypeptide chain, wherein said first chain comprises a biologically active molecule, and at least a portion of an immunoglobulin constant region and

• wherein said second chain consists essentially of at least a portion of an immunoglobulin constant region and optionally an affinity tag.

The chimeric protein of 30, wherein the affinity tag is a FLAG tag.

A chimeric protein comprising a first and second polypeptide chain

• a) wherein said first chain comprises a biologically active molecule, at least a portion of an immunoglobulin constant region, and a first domain having at least one specific binding partner;
• and
• b) wherein said second chain comprises at least a portion of an immunoglobulin without a biologically active molecule or immunoglobulin variable region and further comprising a second domain said second domain being a specific binding partner of said first domain.

A chimeric protein comprising a first and second polypeptide chain

• a) wherein said first chain comprises a biologically active molecule, at least a portion of an immunoglobulin constant region, and a first domain having at least one specific binding partner;
• and
• b) wherein said second chain consists of at least a portion of an immunoglobulin, a second domain said second domain being a specific binding partner of said first domain and optionally an affinity tag.

The chimeric protein of 61, wherein the affinity tag is a FLAG tag.

A chimeric protein comprising a first and second polypeptide chain

• a) wherein said first chain comprises a biologically active molecule, at least a portion of an immunoglobulin constant region, and a first domain having at least one specific binding partner;
• and
• b) wherein said second chain consists essentially of at least a portion of an immunoglobulin, and a second domain said second domain being a specific binding partner of said first domain and optionally an affinity tag.

The chimeric protein of 63, wherein the affinity tag is a FLAG tag.

65-89. (Canceled)

A method of treating a subject with a disease or condition comprising administering a chimeric protein to the subject, such that said disease or condition is treated, wherein said chimeric protein comprises a first and second polypeptide chain,

• a) said first chain comprising an FcRn binding partner, and a biologically active molecule and
• b) said second chain comprising an FcRn binding partner without a biologically active molecule or a variable region of an immunoglobulin.

A chimeric protein of the formula

• X-La-F:F or F:F-La-X
• wherein X is a biologically active molecule, L is a linker, F is at least a portion of an immunoglobulin constant region and, a is any integer or zero.

The chimeric protein of 106, wherein F is an FcRn binding partner.

The chimeric protein of 106, wherein the FcRn is a peptide mimetic of an Fc fragment of an immunoglobulin.

The chimeric protein of 119, wherein the small molecule is a VLA4 antagonist.

A chimeric protein comprising a first and a second polypeptide chain linked together, wherein said first chain comprises a biologically active molecule and at least a portion of an immunoglobulin constant region, and said second chain comprises at least a portion of an immunoglobulin constant region without the biologically active molecule of the first chain and wherein said second chain is not covalently bonded to any molecule having a molecular weight greater than 2 kD.

A chimeric protein comprising a first and a second polypeptide chain linked together, wherein said first chain comprises a biologically active molecule and at least a portion of an immunoglobulin constant region, and said second chain comprises at least a portion of an immunoglobulin constant region not covalently linked to any other molecule except the portion of an immunoglobulin of said first polypeptide chain.

A chimeric protein comprising a first and a second polypeptide chain linked together, wherein said first chain comprises a biologically active molecule and at least a portion of an immunoglobulin constant region, and said second chain consists of at least a portion of an immunoglobulin constant region.

A chimeric protein comprising a first and a second polypeptide chain linked together, wherein said first chain comprises a biologically active molecule and at least a portion of an immunoglobulin constant region, and said second chain comprises at least a portion of an immunoglobulin constant region without the biologically active molecule of the first chain and a molecule with a molecular weight less than 2 kD covalently attached.

154-178. (Canceled)

The method of claim 68, wherein the biologically active molecule is interferon α.

The method of claim 68, wherein the biologically active molecule is interferon β.

The method of claim 168, wherein the chimeric protein comprises interferon α.

The method of claim 168, wherein the chimeric protein comprises interferon β.

A chimeric protein comprising a first and second polypeptide chain, wherein said first chain comprises EPO, an eight amino acid linker having the amino acid sequence EFAGAAAV, and an Fc fragment of an immunoglobulin constant region comprising a mutation of aspargine to alanine at position 297;

• and
• wherein said second chain comprises an Fc fragment of an immunoglobulin constant region comprising a mutation of aspargine to alanine at position 297.

A chimeric protein comprising a first and second polypeptide chain, wherein said first chain comprises IFNβ, an eight amino acid linker having the amino acid sequence EFAGAAAV, and an Fc fragment of an immunoglobulin constant region comprising a mutation of aspargine to alanine at position 297;

• and
• wherein said second chain comprises an Fc fragment of an immunoglobulin constant region comprising a mutation of aspargine to alanine at position 297.

A chimeric protein comprising a first and second polypeptide chain, wherein said first chain comprises factor IX, an eight amino acid linker having the amino acid sequence EFAGAAAV, and an Fc fragment of an immunoglobulin constant region comprising a mutation of aspargine to alanine at position 297;

• and
• wherein said second chain comprises an Fc fragment of an immunoglobulin constant region comprising a mutation of aspargine to alanine at position 297.